Chloroplast DNA variation and pollen contamination in a Pinus brutia Ten. clonal seed orchard: implication for progeny performance in plantations

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dc.authorid0000-0002-3227-6680
dc.authorid0000-0001-8323-2509
dc.authorscopusid22936704800
dc.authorscopusid13409974300
dc.authorwosidbilgen, behiye banu/AAD-9188-2019
dc.authorwosidKaya, Nuray/C-1834-2016
dc.contributor.authorBilgen, Behiye Banu
dc.contributor.authorKaya, Nuray
dc.date.accessioned2022-05-11T14:45:23Z
dc.date.available2022-05-11T14:45:23Z
dc.date.issued2014
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractPollen contamination is one of the important factors affecting the yield, adaptability, and genetic quality of the seed produced from seed orchards in forest tree breeding programs. Incoming pollen from the forests surrounding the seed orchard is a major concern in tree breeding because it contributes to losses in the expected genetic gains from seed orchard crops. The genetic variation and the level of pollen contamination in a 16-year-old Pinus brutia Ten. first-generation clonal seed orchard was studied using chloroplast microsatellite markers (cpSSRs). In total, 23 alleles and 36 unique allelic combinations (haplotypes) were detected based on the 6 cpSSR loci analyzed. The haplotypic diversity of the clones in the seed orchard was found to be 0.849. Out of 300 embryos analyzed, 87 were not compatible with any male parent within the seed orchard. Thus, 29% of the embryos were sired by pollen sources outside the orchard (i.e. apparent contamination). Microsatellite-based analysis revealed that the estimated contamination rate was 39.3%. Background pollination at this level will cause losses of 20% in the expected genetic gains. Our findings are valuable for the assessment of the intended seed orchard function, i.e. provision of genetically improved seed. It may be worthwhile to use pollen management strategies like strobilus stimulation, controlled pollination, and supplemental mass pollination to decrease pollen contamination and increase the genetic quality of the seeds produced.
dc.description.sponsorshipAkdeniz University Scientific Research Project UnitAkdeniz University [2008.03.0121.005]
dc.description.sponsorshipThis study was supported by the Akdeniz University Scientific Research Projects Unit (Project No.: 2008.03.0121.005). We thank Dr Kani Isik (Department of Biology, Faculty of Science, Akdeniz University, Antalya, Turkey) for valuable comments and critical reading of the manuscript. We are grateful to Dr John Frampton (North Carolina State University, NC, USA) and Dr Fikret Isik (North Carolina State University, NC, USA) for reviews and suggestions on the final version the manuscript. Dr Yusuf Kurt, Dr Esref Demir, Dr Ilker Cinbilgel, and Dr Sezgi Seref Gun helped during the field work in the seed orchard and the nearby natural stand. We gratefully acknowledge the contributions of all these persons and institutions.
dc.identifier.doi10.3906/tar-1307-108
dc.identifier.endpage549
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84901218382
dc.identifier.scopusqualityQ2
dc.identifier.startpage540
dc.identifier.urihttps://doi.org/10.3906/tar-1307-108
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10026
dc.identifier.volume38
dc.identifier.wosWOS:000336245400013
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorBilgen, Behiye Banu
dc.language.isoen
dc.publisherTubitak Scientific & Technical Research Council Turkey
dc.relation.ispartofTurkish Journal of Agriculture and Forestry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChloroplast microsatellite locus
dc.subjectgenetic markers
dc.subjectgene flow
dc.subjectclonal seed orchard
dc.subjectTurkish red pine
dc.subjectDouglas-Fir
dc.subjectGene Flow
dc.subjectReproductive Success
dc.subjectMating Patterns
dc.subjectSsr Markers
dc.subjectPopulations
dc.subjectImpact
dc.subjectMicrosatellites
dc.subjectIdentification
dc.subjectPollination
dc.titleChloroplast DNA variation and pollen contamination in a Pinus brutia Ten. clonal seed orchard: implication for progeny performance in plantations
dc.typeArticle

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