Development of doubled haploid maize lines by using in vivo haploid technique
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Tarih
2020
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The doubled haploid technology is now an integral component of modern maizebreeding programs. In this study, the maternal haploid induction (gynogenesis)method was used to derive Doubled-Haploid (DH) lines from elite maize germplasmadapted to Turkey. Temperate haploid inducers (RWS, RWK-76, RWS x RWK-76 andWS14) were used as pollinators, and a set of 30 single-crossses (in FAO 650-700maturity groups) were used as source materials. Putative haploid seeds were selectedbased on expression of R1-nj anthocyanin color marker. Highest haploid induction rate(20.42%) was recorded by using RWK-76 as inducer line, and the lowest haploidinduction rate (17.75%) was obtained through WS14. Putative haploid seeds weregerminated and seedlings were treated with 0.06% colchicine + 0.5%dimethylsulfoxide solution. Following transfer of seedlings into the field, 2178 D0plants were obtained out of a total of 3012 treated haploids. Live plants were from89% of 2178 seedlings which are planted to the field. Fertile plants were formed 57%of live plants. Inbreeding was succeeded in 31.23% of fertile plants and only 7.8% ofinbreeding plants were able to produce seeds. Consequently, 27 doubled haploid lineswere developed.
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Anahtar Kelimeler
Kaynak
Biotech Studies
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Scopus Q Değeri
N/A
Cilt
29
Sayı
1
