Evaluation of genetic diversity and structure of Turkish water buffalo population by using 20 microsatellite markers
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Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI AG
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The present study was aimed to investigate the genetic diversity among 17 Turkish water buffalo populations. A total of 837 individuals from 17 provincial populations were genotyped, using 20 microsatellites markers. The microsatellite markers analyzed were highly polymorphic with a mean number of alleles of (7.28) ranging from 6 (ILSTS005) to 17 (ETH003). The mean observed and expected heterozygosity values across all polymorphic loci in all studied buffalo populations were 0.61 and 0.70, respectively. Observed heterozygosity varied from 0.55 (Bursa (BUR)) to 0.70 (Muş (MUS)). It was lower than expected heterozygosity in most of the populations indicating a deviation from Hardy–Weinberg equilibrium. The overall value for the polymorphic information content of noted microsatellite loci was 0.655, indicating their suitability for genetic diversity analysis in buffalo. The mean FIS value was 0.091 and all loci were observed significantly deviated from Hardy–Weinberg Equilibrium (HWE), most likely based on non-random breeding. The 17 buffalo populations were genetically less diverse as indicated by a small mean FST value (0.032 ± 0.018). The analysis of molecular variance (AMOVA) analysis indicated that about 2% of the total genetic diversity was clarified by population distinctions and 88 percent corresponded to differences among individuals. The information produced by this study can be used to establish a base of national conservation and breeding strategy of water buffalo population in Turkey. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Açıklama
Anahtar Kelimeler
Genetic diversity, Genetic structure, Heterozygosity, Microsatellite loci, Turkish water buffalo, adult, analysis of variance, article, breeding, female, gene frequency, gene structure, genetic variability, heterozygosity, human, human experiment, major clinical study, male, microsatellite marker, nonhuman, synovial bursa, Turkey (republic), water buffalo
Kaynak
Animals
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
11
Sayı
4
