Assessment of the genetic stability of indirect shoot organogenesis-derived plantlets of digitalis trojana ivanina by flow cytometry and cytological analyses

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dc.authorscopusid35789901100
dc.authorscopusid57190797212
dc.authorscopusid52263091800
dc.authorscopusid57225437022
dc.contributor.authorÇördük, N.
dc.contributor.authorYücel, G.
dc.contributor.authorAkıncı, Nihan
dc.contributor.authorTuna, Metin
dc.date.accessioned2022-05-11T14:14:23Z
dc.date.available2022-05-11T14:14:23Z
dc.date.issued2017
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.description.abstractIn this study, flow cytometry and cytological analysis was used to evaluate the genetic stability of Digitalis trojana Ivanina plants regenerated via indirect shoot organogenesis. For in vitro propagation, leaf explants were excised from seedlings grown in sterile conditions and cultured MS medium supplemented with 3.0 mg/L BA + 0.1 mg/L NAA. Shoots and calli were subcultured for a period of 2 weeks for shoot multiplication. For rooting, shoots were separated individually and transferred to MS medium containing 0.1% activated charcoal. Genetic stability of the regenerated plants was assessed by flow cytometry and cytological analyses. Flow cytometric analysis revealed that regenerated plantlets has as 2.80±0.03 pg nuclear DNA (2C) and seed-derived plants has on average 2.80±0.1 pg/2C. Cytological analysis showed that regenerated plantlets have the same number of chromosome with seed-derived plantlets of D. trojana (2n=56). Our results have showed that the plantlets propagated in MS medium with 3 mg/L BA + 0.1 mg/L NAA did not differ genetically from donor plants. Therefore, this system can be effective and suitable for clonal propagation of D. trojana. Our results also confirmed that flow cytometry is fast, easy, accurate and relatively cheap method to determine ploidy of in vitro propagated D. trojana plantlets. © 2017 Namik Kemal University - Agricultural Faculty. All Rights Reserved.
dc.identifier.endpage76
dc.identifier.issn1302-7050
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85044726473
dc.identifier.scopusqualityQ3
dc.identifier.startpage69
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5886
dc.identifier.volume14
dc.indekslendigikaynakScopus
dc.institutionauthorYücel, Gülru
dc.institutionauthorTuna, Metin
dc.language.isoen
dc.publisherNamik Kemal University - Agricultural Faculty
dc.relation.ispartofJournal of Tekirdag Agricultural Faculty
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectC-value
dc.subjectChromosome number
dc.subjectFlow cytometry
dc.subjectFoxglove
dc.subjectNuclear DNA content
dc.subjectSomaclonal variation
dc.titleAssessment of the genetic stability of indirect shoot organogenesis-derived plantlets of digitalis trojana ivanina by flow cytometry and cytological analyses
dc.typeArticle

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