Determination of nuclear dna content and ploidy of some bromus l. Germplasm by flow cytometery

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dc.authorscopusid26430060300
dc.authorscopusid57216248216
dc.authorscopusid57189634048
dc.authorscopusid57225437022
dc.contributor.authorNizam, İlker
dc.contributor.authorGülcü, R.
dc.contributor.authorTuna, G.S.
dc.contributor.authorTuna, M.
dc.date.accessioned2022-05-11T14:02:47Z
dc.date.available2022-05-11T14:02:47Z
dc.date.issued2020
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractThe objective of this study was to determine nuclear DNA content and ploidy of 48 Bromus L. gene bank accessions obtained from USDA by flow cytometry. Based on the results of the flow cytometric analysis, the mean 2C nuclear DNA content of Bromus accessions used in the study varied between 11.43 pg with 26.62 pg. The nuclear DNA content differences among Bromus L. accessions were statistically significant at P<0.01 level. The accessions were easily separated into three distinct groups according to their DNA contents. The mean 2C nuclear DNA content of the groups were 11.82, 22.43 and 26.17 pg (min. and max. values of group were between 11.43–12.65 pg, 21.45–22.77 pg, 25.48–26.62 pg, respectively). Nuclear DNA content of the accessions were correlated with their ploidy levels by counting chromosomes of the one plant from each group with classical staining methods. In conclusion, majority of the Bromus accessions (33 out of 48) analysed in the study were tetraploid with 2n=4x=28 chromosomes. Ten and five of the remaining accessions were octaploid (2n=8x=56) and (2n=10x=70) decaploids, respectively. No decaploid B. inermis plant reported until today. However, decaploids were reported for B. riparius and B. biebersteini species. Therefore, decaploid plants determined in this study should be considered as either B. riparius or B. biebersteini. © 2020, Pakistan Botanical Society. All rights reserved.
dc.description.sponsorshipTOVAG-111O654
dc.description.sponsorshipThis study was supported by a TUBITAK Grant (TOVAG-111O654).
dc.identifier.doi10.30848/PJB2020-3(12)
dc.identifier.endpage913
dc.identifier.issn0556-3321
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85082976094
dc.identifier.scopusqualityQ3
dc.identifier.startpage909
dc.identifier.urihttps://doi.org/10.30848/PJB2020-3(12)
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4482
dc.identifier.volume52
dc.identifier.wosWOS:000522404600020
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorNizam, İlker
dc.institutionauthorGülcü, R.
dc.institutionauthorTuna, G.S.
dc.institutionauthorTuna, M.
dc.language.isoen
dc.publisherPakistan Botanical Society
dc.relation.ispartofPakistan Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBromus L.
dc.subjectFlow cytometry
dc.subjectNuclear DNA content
dc.subjectPloidy
dc.titleDetermination of nuclear dna content and ploidy of some bromus l. Germplasm by flow cytometery
dc.typeArticle

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