Introducing a new method for evaluation of the interaction between an antigen and an antibody: Single frequency impedance analysis for biosensing systems

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dc.authorid0000-0002-5123-5972
dc.authorscopusid56106034900
dc.authorscopusid56106371600
dc.authorscopusid56104725700
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidÖzcan, Burcu/AAY-1451-2021
dc.contributor.authorÖzcan, Burcu
dc.contributor.authorDemirbakan, Burçak
dc.contributor.authorYeşiller, Gülden
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:29:55Z
dc.date.available2022-05-11T14:29:55Z
dc.date.issued2014
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractThis paper illustrates the application of an antibody, anti-parathyroid hormone (anti-PTH), as a bioreceptor in a biosensor system for the first time, and demonstrates how this biosensor can be used in parathyroid hormone (PTH) determination. The interaction between the biosensor and parathyroid hormone was firstly investigated by a novel electrochemical method, single frequency impedance analysis. The biosensor was based on the gold electrode modified by cysteine self-assembled monolayers. Anti-PTH was covalently immobilized onto cysteine layer by using an EDC/NHS couple. The immobilization of anti-PTH was monitored by cyclic voltammetry and electrochemical impedance spectroscopy techniques. The performance of the biosensor was evaluated in terms of linearity, sensitivity, repeatability and reproducibility, after a few important optimization studies were carried out. In particular, parathyroid hormone was detected within a linear range of 10-60 fg/mL. Kramers-Kronig transform was also performed on the impedance data. The specificity of the biosensor was also evaluated. The biosensor was validated by using a complementary reference technique. Lastly the developed biosensor was used to monitor PTH levels in artificial serum samples. (C) 2014 Elsevier B.V. All rights reserved.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109 T 172]
dc.description.sponsorshipWe are thankful for financial support from the Scientific and Technological Research Council of Turkey (TUBITAK, Project number: 109 T 172).
dc.identifier.doi10.1016/j.talanta.2014.02.067
dc.identifier.endpage13
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid24840408
dc.identifier.scopus2-s2.0-84897911146
dc.identifier.scopusqualityQ1
dc.identifier.startpage7
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2014.02.067
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7173
dc.identifier.volume125
dc.identifier.wosWOS:000336872200002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzcan, Burcu
dc.institutionauthorDemirbakan, Burçak
dc.institutionauthorYeşiller, Gülden
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSingle frequency impedance
dc.subjectParathyroid hormone
dc.subjectAnti-parathyroid hormone
dc.subjectBiosensor
dc.subjectParathyroidism
dc.subjectBiomarker
dc.subjectParathyroid-Hormone Secretion
dc.subjectSensing Receptor Expression
dc.subjectGold Surface
dc.subjectX-Rays
dc.subjectSpectroscopy
dc.subjectCalcium
dc.subjectHypoparathyroidism
dc.subjectImmobilization
dc.subjectImmunosensor
dc.subjectDispersion
dc.titleIntroducing a new method for evaluation of the interaction between an antigen and an antibody: Single frequency impedance analysis for biosensing systems
dc.typeArticle

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