Introducing a new method for evaluation of the interaction between an antigen and an antibody: Single frequency impedance analysis for biosensing systems
Yükleniyor...
Dosyalar
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This paper illustrates the application of an antibody, anti-parathyroid hormone (anti-PTH), as a bioreceptor in a biosensor system for the first time, and demonstrates how this biosensor can be used in parathyroid hormone (PTH) determination. The interaction between the biosensor and parathyroid hormone was firstly investigated by a novel electrochemical method, single frequency impedance analysis. The biosensor was based on the gold electrode modified by cysteine self-assembled monolayers. Anti-PTH was covalently immobilized onto cysteine layer by using an EDC/NHS couple. The immobilization of anti-PTH was monitored by cyclic voltammetry and electrochemical impedance spectroscopy techniques. The performance of the biosensor was evaluated in terms of linearity, sensitivity, repeatability and reproducibility, after a few important optimization studies were carried out. In particular, parathyroid hormone was detected within a linear range of 10-60 fg/mL. Kramers-Kronig transform was also performed on the impedance data. The specificity of the biosensor was also evaluated. The biosensor was validated by using a complementary reference technique. Lastly the developed biosensor was used to monitor PTH levels in artificial serum samples. (C) 2014 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Single frequency impedance, Parathyroid hormone, Anti-parathyroid hormone, Biosensor, Parathyroidism, Biomarker, Parathyroid-Hormone Secretion, Sensing Receptor Expression, Gold Surface, X-Rays, Spectroscopy, Calcium, Hypoparathyroidism, Immobilization, Immunosensor, Dispersion
Kaynak
Talanta
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
125
