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Öğe A New Insulin Sensitivity Index Derived From Fat Mass Index and Quantitative Insulin Sensitivity Check Index(Namık Kemal Üniversitesi, Tıp Fakültesi, 2015) Donma, Mustafa Metin; Donma, Orkide; Topçu, Birol; Aydın, Murat; Tülübaş, Feti; Nalbantoğlu, Burçin; Demirkol, Muhammet; Gürel, AhmetAim Obesity has recently become one of the most important health problems throughout the world. This fact led to the controversies on the clinical use of insulin sensitivity indices. Indices previously described or introduced in this study have been evaluated to choose one, which is capable of exhibiting significant distinctions between healthy children and those involved in the classes of childhood obesity. Material and Methods A total of 179 girls; 81 morbidly obese(MO), 42 obese(O), 16 overweight(OW) and 40 normal(N) participated in the study. Groups were constituted based upon age- and sex-specific body mass index percentiles tabulated by World Health Organization. Homeostasis Model Assessment of Insulin Resistance(HOMA-IR), HOMA-IR/BMI, log HOMA-IR, fasting glucose/fasting insulin ratio(FGIR), quantitative insulin sensitivity check index(QUICKI), Raynaud, reciprocal insulin indices and also new indices HOMAIR*BMI, HOMA-IR*fat mass index(FMI), QUICKI*BMI, QUICKI*FMI were calculated. The cut-offs 3.16 and 2.5 for HOMA-IR, 7 and 6 for FGIR, 0.357 and 0.328 for QUICKI were evaluated to estimate insulin resistance. Statistical analyses were performed with Predictive Analytics SoftWare(PASW) Statistics 18. Results and Conclusion QUICKI*FMI was able to make a clear-cut separation between the groups. A new trilogy for cut-offs (HOMA>2.5, FGIR<7, QUICKI<0.328); each giving the similar results, has been suggested. Multifaceted character of QUICKI was also introduced. QUICKI was capable of discriminating MO from O when 0.328 cutoff was used, and O from OW when 0.357 cut-off was used. QUICKI*FMI index, a new one, was unique in detecting the advanced level of differences(p?0.005) between N-OW, OW-O and O-MO groups during childhood obesity.Öğe A post-treatment evaluation of total IgE, CRP and blood count parameters of children with asthma(2013) Tülübaş, Feti; Gürel, Ahmet; Donma, Mustafa Metin; Nalbanto?lu, Burçin; Topçu, Birol; Yıldız, Zeynep DenizObjective: We aimed to study if inhaled corticosteroid and inhaled beta 2 agonist treatment to 2-18 years old children with asthma resulted in recovery in serum total IgE, CRP, and whole blood count levels and/or to evaluate the effect of treatment in comparison to healthy children. Methods: Data regarding pre and post treatment serum total IgE, CRP, and hemogram levels of 2-18 years old, 99 children with asthma, who received inhaled corticosteroid and inhaled beta 2 agonist treatment and who applied to Nami{dotless}k Kemal University Research and Practice Hospital Pediatrics Clinic, were obtained through file scanning. Data regarding demographics and serum total IgE, CRP and hemogram levels of 99 healthy children in control group were also obtained through file scanning. Results: In children with asthma, levels of serum total IgE, lymphocyte, eosinophil and basophil counts and percentages were significantly increased compared to healthy controls, and they were significantly decreased after treatment to the levels of healthy controls. Serum CRP levels, neutrophil counts and percentages were significantly decreased compared to healthy controls, and they were significantly increased after treatment. Conclusions: Inhaled steroid and beta 2 antagonist treatment to children with asthma resulted in recovery in levels of IgE, CRP, and some whole blood count parameters through decreasing inflammation with its anti-inflammatory effect.Öğe An Examination of the Factors Influencing Consumers' Attitudes Toward Social Media Marketing(Routledge Journals, Taylor & Francis Ltd, 2011) Akar, Erkan; Topçu, BirolSocial media and social media applications that build consumer communities involving rich user-generated content are new marketplaces and/or tools for marketers. This study aims to specify the factors affecting consumers' attitudes toward marketing with social media. Given this context, a 7-factor, consumer-based attitude scale that contains 32 items was developed, and a questionnaire was completed by undergraduate students between the ages of 18 and 24. Frequencies, t-test, ANOVA, factor analysis, and regression analysis were used for data analysis. In the end, six factors were found to affect consumers' attitudes toward marketing with social media.Öğe Anatomi Eğitimi Hakkında Öğrenci Görüşlerinin Değerlendirilmesi(Namık Kemal Üniversitesi, Tıp Fakültesi, 2013) Uygur, Ramazan; Çağlar, Veli; Topçu, Birol; Aktaş, Sadiye; Özen, Oğuz AslanAmaç Çalışmamızda Namık Kemal Üniversitesi Tıp Fakültesi’nde eğitim gören ikinci sınıf öğrencilerinin Anatomi Anabilim Dalı tarafından verilen anatomi eğitiminin mevcut durumu hakkındaki görüşlerinin yapılan anket yardımıyla değerlendirilmesi amaçlandı. Materyal ve Metot Çalışmamıza 2012-2013 eğitim-öğretim yılında Namık Kemal Üniversitesi Tıp Fakültesi ikinci sınıfta anatomi dersini alan 79 öğrenci dahil edildi. Öğrencilerin almış oldukları anatomi eğitimi hakkındaki görüşleri anket yöntemiyle belirlendi. Bulgular Çalışmamızda öğrencilerin tıp fakültelerini isteyerek tercih ettikleri ve anatomi eğitiminden memnun oldukları görüldü. Öğrencilerin sınıf mevcudunun az olmasını, uygulama dersi çalışma gruplarının daha az sayıda kişiden oluşmasını, kadavra ve maket sayısının arttırılmasını, uygulama ders saatinin arttırılmasını istedikleri belirlendi. Öğrencilerin anatomi sınavlarının sayı ve formatını uygun buldukları, ders içerisinde verilen klinik anatomi bilgilerinden memnun oldukları saptandı. Anatomi dersinde başarılı olan öğrencilerin derse daha ilgili, ders çalışmayı seven, derse devamlılık gösteren kişiler oldukları ve öğrendikleri anatomi bilgilerini kullanabildikleri tespit edildi. Sonuç Anatomi eğitiminden daha fazla verim alabilmek için, öğrencilerin anatomi dersi hakkındaki görüşlerinin alınmasının anatomi eğitimine katkı sağlayabileceği ve yol gösterici nitelikte olabileceği belirlendi.Öğe Antioxidant and anti-apoptotic effects of onion (Allium cepa) extract on doxorubicin-induced cardiotoxicity in rats(Wiley, 2013) Alpsoy, Şeref; Aktaş, Cevat; Uygur, Ramazan; Topçu, Birol; Kanter, Mehmet; Erboğa, Mustafa; Gedikbaşı, AsumanThe aim of this study was to investigate the antioxidant and anti-apoptotic effects of onion (Allium cepa) extracts (ACE) on doxorubicin (DOX)-induced cardiotoxicity. The rats in the ACE-pretreated group were given a daily dose of 1 ml ACE for 14 days. To induce cardiotoxicity, DOX (30 mg kg-1 body weight) was injected intraperitoneally by a single dose and the rats were sacrificed after 48 h. To date, no such studies have been performed on the cardioprotective and anti-apoptotic potential of ACE on DOX-induced cardiotoxicity. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end-labeling in cardiomyocytes of the DOX-treated group with ACE therapy. The DOX-treated with ACE groups showed a significant decrease in malondialdehyde levels, and increased activities of superoxide dismutase, glutathione and glutathione peroxidase in comparison with the DOX-treated group. Creatine kinase, creatine kinase MB, lactate dehydrogenase activities and cardiac troponin I levels were significantly decreased in the DOX + ACE group in comparison with the DOX group. These biochemical and histological disturbances were effectively attenuated on pretreatment with ACE. The present study showed that ACE may be a suitable cardioprotector against toxic effects of DOX. Copyright (C) 2011 John Wiley & Sons, Ltd.Öğe Assessment of Nursing and Technical Sciences Students' Perceptions of the Health Locus of Control(2019) Önler, Ebru; Yıldız, Tülin; Topçu, Birol; Bahar, Semiha; Güler, Mehmet CanAim: This research aimed to assess the perception of the health locus of control in nursing and technical sciencesstudents.Methods: The study conducted at one state university in 2014 and consisted of 413 (192 nursing, 222 technicalsciences) students. Data were obtained with the Multidimensional Health Locus of Control Scale, and a surveyabout the socio-demographic and some of the health behaviors of students and evaluated with percentage,frequency, median, standard deviation, ANOVA, t and Tukey tests.Results: The school type of students were observed to affect their health locus of control perceptions. Studentswho exercised regularly had more internal control, whereas students who do not drink alcohol were moredeterministic in terms of their health.Conclusion: Students should be encouraged to improve a sense of responsibility in health and disease, andenhance internal health locus of control.Öğe Association of colon adenomas and skin tags: coincidence or coexistence?(Verduci Publisher, 2014) Oran, Mustafa; Erfan, Gamze; Mete, Rafet; Yanık, Mehmet Emin; Yıldırım, Oğuzhan; Aydın, M.; Topçu, BirolOBJECTIVES: Skin tag (STs) are benign connective tissue tumors of the dermis. Some researchers have argued that there is a relationship between skin tag and colon polyps, although the physiopathological mechanisms underlying this relation were not well elucidated. In this study we aimed to investigate the co-existence of colonic adenomatous polyps and ST, additionally to shed light on the physiopathological mechanisms underlying this coincidence. PATIENTS AND METHODS: A total of 45 patients aged between 18 and 60 diagnosed with adenomatous colonic polyps and 45 sex, age, and socio-demographically matched subjects, had no polyps, were enrolled as the control group. Routine blood analysis of all participants, including serum glucose, total cholesterol, low-density lipoprotein cholesterol (LDL-C), (high-density lipoprotein cholesterol (HDL-C), triglyceride, insulin, IGF-1, and EGF, were performed. The chi-square and independent sample t or Mann Whitney U test were used to determine differences between groups. RESULTS: The number of participants with ST was significantly higher in the patient group (OR 7.067, p < 0.01). Serum levels of IGF-1 and EGF were statistically similar between the groups. In the subgroup analyses, no difference was found in serum levels of insulin, IGF-1, or EGF between patients with and without ST. However, higher serum levels of insulin and EGF were found in control subjects with ST (p < 0.01 and p < 0.01, respectively). For the entire study group, 67 participants had ST and 23 patients did not. Serum insulin, and IGF-1 were similar, while serum EGF levels were higher in patients with ST (p < 0.01). CONCLUSIONS: Findings of the present study may show a co-existence of colonic polyps and ST. Although previous studies have indicated that insulin resistance may play a role in the pathogenesis of both lesions in diabetic and obese patients, we found no indication of a relationship in nondiabetic and nonobese patients with increased levels of EGF in patients with ST, suggesting an alternative pathogenesis in this patient group.Öğe Astma ve alerjik rinitli çocuklarda total IgE, C-reaktif protein ve kan sayım parametrelerinin değerlendirilmesi(2013) Tülübaş, Feti; Gürel, Ahmet; Donma, Mustafa Metin; Nalbantoğlu, Burçin; Topçu, Birol; Mut, Zeynep DenizAmaç: Bu çalışmanın amacı; astım ve alerjik rinit tanısı alan çocukların total IgE, C reaktif protein (CRP) ve tamkan sayım parametrelerinin hastalık ile ilişkilerinin olup olmadığını retrospektif olarak araştırmaktır.Gereç ve Yöntem: Hastanemiz Çocuk Sağlığı ve Has- talıkları polikliniğine gelen 2-18 yaş arası toplam 443 ço- cuğun retrospektif taraması yapıldı. Muayene sonuçları- na göre çocuklar astım (n=179), alerjik rinit (n=171) ve kontrol grubu (n=93) olarak üçe ayrıldı. Her gruptaki ço- cukların serum total IgE ve CRP düzeyleri ile hemogram değerleri, yaş ve cinsiyetleri kaydedildi. Bulgular: Astım grubunda eozinofil sayısı, MCHC ve total IgE düzeyleri kontrol grubuna göre anlamlı olarak artar- ken, MCV düzeyi azalmıştır. Alerjik rinit grubunda lenfosit sayısı, CRP ve total IgE düzeyleri kontrol grubuna göre anlamlı olarak artarken nötrofil sayısı azalmış, eozinofil sayısında ise anlamlı bir değişiklik gözlenmemiştir. Sonuçlar: Bu sonuçlar; astım patagonezinde etkili olan faktörlerin eritrosit morfolojisi üzerine de etkili olduğunu düşündürmektedir. Ayrıca, astımda kan eozinofil düzeyin- deki değişiklikler, alerjik rinitte ise nötrofil ve lenfosit düze- yindeki değişikler dikkat çekmekte olup, astımda serum total IgE düzeylerinde, alerjik rinitte ise CRP düzeylerinde artış saptanmıştır.Öğe Asymmetric dimethylarginine as risk marker of endothelial dysfunction in obese children(Wiley-Blackwell, 2016) Aydın, M.; Donma, Mustafa Metin; Donma, Orkide; Nalbantoğlu, Burçin; Tülübaş, Feti; Topçu, Birol; Gürel, A.; Demirkol, Muhammet; Yılmaz, A.; Küçükyalçın, V.[No Abstract Available]Öğe Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD (Meeting Abstract)(European Respiratory Soc Journals Ltd, 2015) Aydın, Murat; Altıntaş, Nejat; Mutlu, Levent Cem; Bilir, Bülent; Oran, Mustafa; Tukubas, Feti; Topçu, Birol[No Abstract Available]Öğe Atherosclerosis, some serum inflammatory markers in psoriasis(Edizioni Minerva Medica, 2014) Alpsoy, Şeref; Akyuz, A.; Erfan, Gamze; Akkoyun, Dursun Çayan; Topçu, Birol; Güzel, Savaş; Kulaç, MustafaAim. Psoriasis is an autoimmune inflammatory disease which is associated with increased inflammatory markers and atherosclerosis. We wanted to investigate whether there is a relationship between some inflammatory markers and subclinical atherosclerosis markers. Methods. We studied 60 psoriasis patients and 50 healthy controls. Demographic, biochemical parameters, C3, C4, d-dimer, CRP, fibrinogen and YKL-40 (human cartilage glyco-protein-39) levels were measured. After measuring carotid intima-media thickness (cIMT) and aortic elasticity parameters such as aortic strain, (beta) stiffness index and compliance, statistical comparisons were done. Results. Patients with psoriasis had significantly higher diastolic blood pressure, CRP, fibrinogen, C3, uric acid levels, beta-stiffness index, and cIMT values than the control group. cIMT was correlated with CRP, YKL-40 and psoriasis area and severity index (PASI) score (r=0.219, P=0.038; r=0.225, P=0.033 and r=0.275, P=0.034). Aortic strain (%), aortic compliance and aortic stiffness index were correlated with C3 (r=-0.349, r=-0.526 and r=0.235) and fibrinogen (r=-0.354, r=-0.275 and r=0.289), all P values <0.05, but not with PASI score. The presence of psoriasis was related to aortic strain (beta +/- SE: -2.055 +/- 0.861, P=0.019) and beta-stiffness index (beta +/- SE: 2.934 +/- 1.143, P=0.012). Conclusion. Serum C3, CRP, fibrinogen and YKL-40 levels are elevated as well as increased cIMT and impaired aortic elasticity in psoriasis. CRP, YKL-40 and PASI score are correlated with cIMT. Increased serum C3 and fibrinogen levels correlate negatively with aortic strain and aortic compliance, and correlate positively with the beta-stiffness index.Öğe B-mode ultrasound assessment of carotid artery structural features in patients with normocalcaemic hyperparathyroidism(Via Medica, 2023) Elbüken, Gülşah; Aydın, Cihan; Öztürk, Beyza Olcay; Aykaç, Hüseyin; Topçu, Birol; Zuhur, Sayid ShafiIntroduction: Normocalcaemic hyperparathyroidism is a condition first defined in 2008, characterized by normal se- rum calcium and high parathormone levels. Although normocalcaemic hyperparathyroidism is considered to have a milder clinical picture compared to asymptomatic primary hyperparathyroidism, recent studies have shown that it may be associated with osteoporosis, insulin resistance, metabolic syndrome, and cardiovascular risk factors. Considering that normo- calcaemic hyperparathyroidism may pose a cardiovascular risk in the setting of carotid atherosclerosis, we sought to examine the structural features of the carotid artery in patients with normocalcaemic hyperparathyroidism compared to a control group. Material and Methods: After excluding patients with hypertension, diabetes, and dyslipidaemia (other factors contributing to atheroscle- rosis), 37 (32 females, 5 males) patients with normocalcaemic hyperparathyroidism with a mean age of 51.2 +/- 8 (min: 32, max: 66) years and 40 controls (31 females, 9 males) with a mean age of 49.3 +/- 7.5 (min: 34, max: 64) years with normal serum albumin-corrected calcium and parathyroid hormone levels were included in the study. Structural features of the carotid artery including intima-media thickness (mean and maximum), lumen diameter, and the presence of plaque were assessed using B-mode ultrasound. Results: On ANCOVA analysis corrected for atherosclerotic factors (body mass index, waist circumference, fasting plasma glucose, serum cholesterol, lipid, and blood pressure), greater mean intima-media thickness was found in patients with normocalcaemic hyperparathyroidism than in controls (0.65 mm vs. 0.59 mm, respectively) (p = 0.023). Maximum ca- rotid intima-media thickness was also greater in patients with normocalcaemic hyperparathyroidism compared to controls (0.80 mm vs. 0.75 mm, respectively) (p = 0.044). The study groups did not show a significant difference in lumen diameter and the presence of carotid plaque. In addition, a negative correlation was found between parathormone (PTH) level and lumen diameter. Conclusion: The findings of this study show that as with asymptomatic primary hyperparathyroidism, normocalcaemic hyperparathy- roidism may be associated with increased cardiovascular risk by predisposing to atherosclerosis. (Endokrynol Pol 2023; 74 (1): 67-73)Öğe Blood Pressures may be Predictor of Cardiac Ischemia in Myocardial Perfusion Scintigraphy(2020) Demirkıran, Aykut; Çekirdekçi, Elif İjlal; Topçu, Birol; Yavuz, Hacite SümeyyeBlood Pressures may be Predictor of Cardiac Ischemia in Myocardial Perfusion Scintigraphy Aykut Demirkıran1, Elif İjlal Çekirdekçi2, Birol Topçu3, Hatice Sümeyye Yavuz41Department of Cardiology, Tekirdag State Hospital, Tekirdag2Department of Cardiology, University of Kyrenia, Kyrenia3Department of Medical Biostatistics, Namik Kemal University, Tekirdag4Department of Nuclear Medicine, Tekirdag Corlu State Hospital, TekirdagAbstractDOI: 10.5543/khd.2020.77699 Turk J Cardiovasc Nurs 2020;11(26):105–110Kardiyovasküler Hemşirelik DergisiObjective: Low diastolic blood pressure (DBP) is associated with adverse cardiovascular outcomes in patients with chronic coronary disease (CAD), but its association with ischemia is unknown. Relationship between DBP and the myo-cardial ischemia in myocardial perfusion scintigraphy (MPS) was investigated.Methods: Patients with chronic coronary artery disease who underwent MPS were included. One day stress/rest gated MPI with Tc-99m MIBI protocol was applied to all patients. Blood pressures was measured before MPI. Patients were divided into 2 groups as ?75 mmHg and >75 mmHg according to DBP. The SDS were calculated using the sum of the 17-segment.Results: The patients with DBP ?75 mmHG and with DBP>75 mmHG were compared, there was no significant differ-ence in SDS (4.44±4.67 and 4.65±4.70 respectively; p=0.657). Association of DBP ?75 mmHg with SDS appeared to be primarily among those with SBP >130 mmHg. Patients with DBP ?75mmHg and SBP >130mmHg had different SDS (6.87±6.00 and 4.99±4.77; p=0.015) between patients with DBP >75 mmHg and SBP >130mmHg. Conclusion: Coexistence lower diastolic blood pressures (?75mmHg) with higher systolic blood pressures (>130mmHg) could be a predictor of myocardial ischemia in patients who underwent adenosine stress MPIÖğe Caga Strain of Helicobacter Pylori in Recurrent Aphtous Somatitis Patients Without Dyspeptic Symptoms: Response to Eradication Therapy(Carbone Editore, 2014) Erfan, Gamze; Mete, Rafet; Oran, Mustafa; Yanık, Mehmet Emin; Güneş, Hayati; Kaya, Şule; Topçu, BirolHelicobacter pylori (HP) play a role as an etiological factor of soine dermatological diseases such as rosacea and Behcet disease. Recurrent aphtus stomatitis (RAS) is the most common mucosal disease with unknown etiology. Several microbial agents such as HP also have been proposed as causative factors for this disease. Although some of the studies do not support a strong association between HP and RAS, the relationship might differ when the virulence of the infecting strains are examined. The cytotoxin-associated gene A (CagA) is one of the virulence factors of HP and has impact in some of the extra gastrointestinal dermatological diseases such as rosacea. In this study; the seroprevelance of CagA of non-dyspeptic RAS patients and the eradication treatment response in CagA positive and negative RAS patients were examined. In 87 RAS patients and 72 healthy volunteers; 43.6% of RAS patients and 25% of control subjects were CagA (+). The number of CagA (+) patients were significantly higher in RAS group. (OR: 3.61, 95%CI: 1.21-10.7, p=0.01). Out of 45 HP (+); 32 (80%) of patients who underwent eradication treatment assessed three months after treatment. In CagA (+) RAS patients; composite index score of RAS after eradication was significantly lower than before treatment (p 0.01) but in CagA (-) RAS patients statistical analysis revealed no difference (p= 0.11). The CagA strain of HP is significantly higher even in non-dyspeptic RAS patients than healthy cases. The eradication treatment in these patients with HP and CagA seropositivity improves symptoms of RAS.Öğe Can low brain-derived neurotrophic factor levels be a marker of the presence of depression in obese women?(Dove Medical Press Ltd, 2014) Güzel, Eda Çelik; Bakkal, Esra; Güzel, Savaş; Eroğlu, Hasan Emre; Acar, Ayşe; Küçükyalçın, Volkan; Topçu, BirolObjective: Depression is a common condition in obese women that can result in severe impairment of their physical and social functioning. A deficiency of brain-derived neurotrophic factor (BDNF) is involved in the mechanism of depression. The aim of this study is to investigate whether BDNF levels differ between obese female patients and healthy controls and whether BDNF levels alter with affective states in depressive obese women. Methods: The study group included 40 obese, 40 preobese, and 40 normal weight women. BDNF levels were measured with an enzyme-linked immunosorbent assay in patient and control groups. For identifying the depression and anxiety status, Beck Depression/Anxiety Inventories were used; and for the evaluation of cognitive functions, the mini-mental state examination was used. Results: BDNF levels were significantly lower in obese patients compared to the control group (P. 0.01). BDNF levels were significantly lower in obese patients with depression compared to the obese patients without depression (P<0.05). The Beck Depression Inventory showed a negative correlation with BDNF (r=-0.044; P<0.01) and a positive correlation with the Beck Anxiety Inventory (r= 0.643; P<0.001), vitamin B12 levels (r= 0.023; P<0.001), and insulin levels (r= 0.257; P<0.05) in obese patients. When receiver operating characteristic curve analysis was used to analyze the suitability of BDNF to identify depression in obese women, the area under the curve for BDNF, 0.756, was found to be significant (P=0.025). BDNF levels lower than 70.2 pg/mL were associated with a higher prevalence of depressive symptoms. Conclusion: The results of our study suggest that the decrease in BDNF levels can be used as a marker for depression diagnosis in obese patients.Öğe Can the development of atrial fibrillation in patients with ischemic heart failure with low ejection fraction be predicted?(Kare Publ, 2020) Alpsoy, Şeref; Erselcan, Kubilay; Akyüz, Aydın; Özkaramanlı Gür, Demet; Topuz, Sahin; Topçu, Birol; Güler, NiyaziOBJECTIVE: Our aim is to determine the triggering factors of paroxysmal atrial fibrillation (PAF) in ischemic heart failure (HF) patients with low ejection fraction (EF). METHODS: Sixty patients were included in this study. Echocardiography and 24-hours Holter monitoring were performed after measurement of serum NT-pro BNP concentration. The patients were classified into two groups concerning the occurrence of PAF on Holter recordings. Biochemical and echocardiographic parameters of patients with and without PAF were compared. RESULTS: PAF was detected in 28 (46%) patients. Patients with PAF demonstrated higher NT-pro BNP levels, mitral and aortic regurgitation velocities, E/A, E/E', pulmonary capillary wedge pressure, pulmonary artery systolic pressure, left atrial volume and volume indices. NT-pro BNP was established as the predictor of PAF (OR=1.23, 95% CI: 1.08-1.42; p=0.001).ROC analysis showed an NT-pro BNP value of 2188 pg/mL as cut-off value with 68% sensitivity and 84% specificity [Area under the ROC curve (AUC)=0.826, CI 95%: 0.724-0.927; p<0.001]. CONCLUSION: The triggering factors for AF are increased intracardiac pressures, left atrial dilatation and increased wall tension. As an indicator of increased wall tension, elevated levels of NT-pro BNP predict the development of PAF.Öğe Candida albicans ve Candida parapsilosis Kompleks İzolatlarının Biyofilm Üretimi Üzerine Farnesol Etkisinin Araştırılması(2024) Erdal, Berna; Baylan, Bensu; Batar, Bahadır; Öztürk, Ali; Topçu, BirolCandida türlerinin neden olduğu enfeksiyonların insidansı son otuz yılda önemli ölçüde artmıştır. Kandidozda birinci sırada Candida albicans izole edilirken, Candida parapsilosis gibi non-albicans Candida türleri de sıklıkla patojen olarak tanımlanmaktadır. Candida enfeksiyonlarının patogenezinde etkili mekanizmalardan biri olan biyofilm oluşumu nedeniyle tedaviye dirençli enfeksiyonlar ortaya çıkmaktadır. Çoğunluğu algılama [quorum sensing (QS)] sistemi molekülü olan farnesolün Candida türlerinin biyofilm yapımı üzerine etki mekanizmaları halen belirsizliğini korumaktadır. Bu çalışmada, C.albicans ve C.parapsilosis kompleks izolatlarının farnesol varlığında biyofilm biyokütlesindeki değişiklikler ile QS moleküllerinin sentezinde görev aldığı düşünülen EFG1 ve BCR1 genlerinin kantitatif ters transkriptaz polimeraz zincir reaksiyonu [quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR)] ile gösterilmesi amaçlanmıştır. Çalışmaya farklı klinik örneklerden izole edilen C.albicans (n= 91) ve C.parapsilosis kompleks (n= 29) izolatları dahil edilmiştir. Farnesolün minimum inhibitör konsantrasyon (MİK) değerleri Klinik ve Laboratuvar Standartları Enstitüsü [Clinical Laboratory Standards Institution (CLSI)] M27-A3 kılavuzu kullanılarak sıvı mikrodilüsyon yöntemiyle belirlenmiştir. İzolatların farnesolsüz, farnesolün MİK-0 ve MİK-2 konsantrasyonlarındaki biyofilm biyokütleleri incelenmiştir. Biyofilm ile ilişkili EFG1 ve BCR1 genlerinin ifadelerindeki değişiklikler qRT-PCR ile araştırılmıştır. Çalışma sonucunda, C.albicans izolatlarının %82.4 (n= 75)’ünde farnesol MİK değerleri 1-2 mM aralığındaki konsantrasyonlarda saptanırken, C.parapsilosis kompleks izolatlarının %72.4 (n= 21)’ünde 0.5-1 mM aralığında bulunmuştur. C.albicans izolatlarının %29.7 (n= 27)’si kuvvetli biyofilm, %63.7 (n= 58)’si zayıf biyofilm oluştururken, C.parapsilosis kompleks izolatları için bu oranların sırasıyla %34.4 (n= 10) ve %62.1 (n= 18) olduğu bulunmuştur. Kuvvetli biyofilm oluşturan C.albicans (n= 24, %88.9) ve C.parapsilosis kompleks (n= 8, %80.0) izolatlarında farnesolün MİK-0 ve MİK-2 konsantrasyonlarının biyofilm biyokütlesini azalttığı, zayıf biyofilm oluşturan C.albicans (n= 35, %60.3) ve C.parapsilosis kompleks (n= 10, %55.6) izolatlarında ise arttırdığı gözlenmiştir. Biyofilm deneyi sonuçlarını destekleyen qRT-PCR çalışması sonucunda; kuvvetli biyofilm yapan C.albicans ve C.parapsilosis kompleks izolatlarında farnesolün MİK-0 ve MİK-2 konsantrasyonlarında EFG1 ve BCR1 gen ifadelerinde azalış olduğu ancak zayıf biyofilm yapan izolatlarda gen ifadelerinde artış olduğu belirlenmiştir. Bu çalışma farnesolün Candida türleri üzerine antifungal etkisine ek olarak farnesolün MİK-0 ve MİK-2 konsantrasyonlarının Candida biyofilm biyokütlesine karşı etkinliğine ait veriler sunmaktadır. Sonuçlar, farnesolün Candida enfeksiyonlarında biyofilm oluşumunu azaltmak için alternatif bir ajan olarak kullanılabileceğini düşündürse de yapılacak başka çalışmalar ile bu sonuçların desteklenmesi gerekmektedir. Ayrıca bu çalışma farnesol varlığında C.parapsilosis kompleks izolatlarının EFG1 ve BCR1 gen ifadelerini belirlemek adına yapılan ilk çalışma olması açısından önemlidir.Öğe Cardioprotective effects of fish omega-3 fatty acids on doxorubicin-induced cardiotoxicity in rats(Sage Publications Ltd, 2014) Uygur, Ramazan; Aktaş, Cevat; Tülübaş, Feti; Alpsoy, Şeref; Topçu, Birol; Özen, Oğuz AslanThe aim of this study was to investigate the protective effects of fish omega-3 (n-3) fatty acids on doxorubicin (DOX)-induced acute cardiotoxicity. A total of 24 rats were divided into three groups: control, DOX-treated, and DOX treated with fish n-3 fatty acids. Control group received 0.4 ml/kg/day of saline intragastrically. The rats in the fish n-3 fatty acid-pretreated group were given 400 mg/kg/day fish n-3 fatty acids for 30 days by intragastric intubation. To induce acute cardiotoxicity, DOX (30 mg/kg) was injected intraperitoneally by a single dose and the rats were killed after 48 h. DOX treatment caused severe damage in heart tissues. Disorganization of myocardial muscle fibers, myofibrillar loss, and cardiotoxic myocardial fibers with cytoplasmic vacuoles were seen. Fish n-3 fatty acid-treated rats showed an improved histological appearance in the DOX-treated group. Our data indicate a significant reduction in the activity of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling in cardiomyocytes of the DOX-treated group with fish n-3 fatty acids therapy. The DOX-treated with fish n-3 fatty acids group showed a significant decrease in malondialdehyde levels, and an increase in superoxide dismutase and glutathione peroxidase activities in comparison with the DOX-treated group. This study showed that fish n-3 fatty acids may be a suitable cardioprotector against acute toxic effects of DOX.Öğe Carotid arteries and vertebrobasilary system intracranial stenosis correlates with multi vessel coronary artery disease(Comenius Univ, 2019) Özçağlayan, Ömer; Kurtoğlu Özçağlayan, Tuğba İlkem; Özkaramanlı Gür, Demet; Gumusel, H. K.; Topçu, Birol; Ünal, A.BACKGROUND: The goal of this study was to show the importance of the identifying potential carotid and vertebrobasilar stenosis with Computed Tomography Angiography (CTA) in severe coronary artery disease. METHODS: In 109 patients, CTA of the carotid and the vertebrobasilar system were taken in the six months following the Coronary Angiography (CA). Coronary arteries and carotid vertebrobasilar system stenosis were considered significant at more than >= 50 %. RESULTS: A significant statistical relationship was found between a coronary artery group of three-vessel disease (3-VD) and stenosis of the cervical segments of the right (p = 0.022) and left internal carotid artery (ICA) (p = 0.001); intracranial segments of the right (p = 0.007) and left ICA (p = 0.020), and the right vertebral artery (VA) (p = 0.008). There was a significant statistical relationship between Gensini score and stenosis of both the right (p = 0.030) and the left ICA cervical segments (p = 0.003). CONCLUSION: In patients with severe coronary artery disease especially in 3-VD, CTA scan may be useful diagnostic tool for identifying stenosis of the carotid arteries, particularly in the intracranial segments of the ICA and in the preforaminal (V1) segment of the VAÖğe CD4(+), CD25(+), FOXP3(+) T Regulatory Cell Levels in Obese, Asthmatic, Asthmatic Obese, and Healthy Children(Springer/Plenum Publishers, 2015) Donma, Mustafa Metin; Karasu, Erkut; Özdilek, Burcu; Turgut, Burhan; Topçu, Birol; Nalbanto?lu, Burçin; Donma, OrkideThe aim of this prospective case control study is to determine CD4(+), CD25(+), and FoxP3(+) T regulatory cells (Tregs) and T helper cells (Ths) in obese, asthmatic, asthmatic obese, and healthy children. Obese (n = 40), asthmatic (n = 40), asthmatic obese (n = 40), and healthy children (n = 40) were included in this study. Blood samples collected from children were marked with CD4, CD25, ve Foxp3 in order to detect Tregs and Ths by flow cytometric method. Statistical analyses were performed. p a parts per thousand currency signaEuro parts per thousand 0.05 was chosen as meaningful threshold. Tregs exhibiting anti-inflammatory nature were significantly lower in obese (0.16 %; p a parts per thousand currency signaEuro parts per thousand 0.001), asthmatic (0.25 %; p a parts per thousand currency signaEuro parts per thousand 0.01), and asthmatic obese (0.29 %; p a parts per thousand currency signaEuro parts per thousand 0.05) groups than control group (0.38 %). Ths were counted higher in asthma group than control (p a parts per thousand currency signaEuro parts per thousand 0.01) and obese (p a parts per thousand currency signaEuro parts per thousand 0.001) groups. T cell immunity plays important roles in chronic inflammatory diseases such as obesity and asthma pathogeneses. Decreased numbers of Tregs found in obese, asthmatic, and asthmatic obese children might represent a challenge of these cells.
