Ultrasensitive Impedimetric Biosensor Fabricated by a New Immobilisation Technique for Parathyroid Hormone

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dc.authorid0000-0002-7653-6480
dc.authorid0000-0002-6232-3388
dc.authorscopusid23101201700
dc.authorscopusid57209066492
dc.authorscopusid56638673200
dc.authorscopusid24553843800
dc.authorscopusid25935968900
dc.authorscopusid6603077608
dc.authorscopusid6506764918
dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.authorwosidAYDIN, TUBA/W-4227-2017
dc.authorwosidAsav, Engin/AAG-1170-2021
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidAsav, Engin/AAG-4182-2021
dc.contributor.authorÖzcan, Hakkı Mevlüt
dc.contributor.authorYıldız, Kübra
dc.contributor.authorÇakar, Cansu
dc.contributor.authorAydın, Tuba
dc.contributor.authorAsav, Engin
dc.contributor.authorSağıroğlu, Ayten
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:30:53Z
dc.date.available2022-05-11T14:30:53Z
dc.date.issued2015
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractThis paper presents a novel ultrasensitive and rapid impedimetric biosensor with new immobilisation materials for parathyroid hormone (PTH) with the aim to determine the PTH level in serum for the diagnosis and monitoring of parathyroid diseases such as hyperparathyroidism, adenoma, and thyroid cancer. The interaction between PTH and the biosensor was investigated with an electrochemical method. The biosensor was based on the gold electrode modified by mercaptohexanol (6-MHL). Anti-parathyroid hormone (anti-PTH) was covalently immobilised onto a self-assembled monolayer (SAM) by using epiclorhidrina (EPI) with ethanolamine (EA). The EPI-EA interaction represents the first use of these for the construction of biosensors in published reports. The immobilisation of the anti-PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscopy (SEM) techniques. After the optimisation studies of immobilisation materials such as 6-MHL, EPI, EA and glutaraldehyde, linearity, repeatability and sensitivity of biosensor were evaluated as the performance of biosensor. PTH was detected within a linear range of 0.1-0.6 pg/ml, and the detection limit was 0.1 fg/ml. The specificity of the biosensor was also investigated. Finally, the described biosensor was used to detect the PTH levels in artificial serum samples.
dc.description.sponsorshipTrakya University Scientific Research FoundationTrakya University [TUBAP-2001/195]; TUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipSupport from the Trakya University Scientific Research Foundation (project number: TUBAP-2001/195) and TUBITAK (The Scientific and Technological Research Council of Turkey; project number: 113 Z 678) are greatly acknowledged.
dc.identifier.doi10.1007/s12010-015-1643-x
dc.identifier.endpage1262
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue5en_US
dc.identifier.pmid25935225
dc.identifier.scopus2-s2.0-84937971512
dc.identifier.scopusqualityQ2
dc.identifier.startpage1251
dc.identifier.urihttps://doi.org/10.1007/s12010-015-1643-x
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7214
dc.identifier.volume176
dc.identifier.wosWOS:000358379400002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectParathyroid hormone
dc.subjectBiosensor
dc.subjectEpiclorhidrina
dc.subjectElectrochemical impedance spectroscopy
dc.subject6-mercaptohexanol
dc.subjectSelf-Assembled Monolayers
dc.subjectImpedance Biosensor
dc.subjectProtein
dc.subjectSam
dc.titleUltrasensitive Impedimetric Biosensor Fabricated by a New Immobilisation Technique for Parathyroid Hormone
dc.typeArticle

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