Purification and Biochemical Characterization of a Novel Thermostable Serine Protease from Geobacillus sp. GS53
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Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Proteases account for approximately 60% of the enzyme market in the world, and they are used in various industrial applications including the detergent industry. In this study, production and characterization of a novel serine protease of thermophilic Geobacillus sp. GS53 from Balçova geothermal region, İzmir, Turkey, were performed. The thermostable protease was purified through ammonium sulfate precipitation and anion-exchange chromatography. The results showed that the protease had 137.8 U mg?1 of specific activity and optimally worked at 55 oC and pH 8. It was also active in a broad pH (4–10) and temperature (25–75 °C) ranges. The protease was highly stable at 85 °C and demonstrated relative stability at pH 4, 7, and 10. Also, the enzyme had high stability against organic solvents and surfactants; enzyme relative activity did not decrease below 81% upon preincubation for 10 min. Ca2+, Cu2+, and Zn2+ ions slightly induced protease activity. The protease was highly specific to casein, skim milk, Hammerstein casein, and BSA substrates. These results revealed that the protease might have a potential effect in a variety of industrial fields, especially the detergent industry, because of its high thermostability and stability to surfactants. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.
Açıklama
Anahtar Kelimeler
Geobacillus, Serine protease, Surfactant, Thermostability, Amino acids, Casein, Dairies, Nitrogen compounds, Purification, Sulfur compounds, Surface active agents, Ammonium sulfate precipitation, Anion exchange chromatography, Biochemical characterization, Detergent industry, Industrial fields, Protease activities, Relative activities, Relative stabilities, Enzyme activity, alkaline proteinase, ammonium sulfate, calcium ion, casein, cuprous ion, serine proteinase, zinc ion, calcium, copper, serine proteinase, surfactant, zinc, anion exchange chromatography, Article, bacterial strain, biochemistry, controlled study, enzyme activity, enzyme purification, enzyme specificity, enzyme stability, Geobacillus, Geobacillus stearothermophilus, Geobacillus toebii, ion exchange chromatography, molecular weight, nonhuman, pH, polyacrylamide gel electrophoresis, precipitation, skim milk, temperature, Turkey (republic), chemistry, enzyme stability, enzymology, Geobacillus, metabolism, Calcium, Chromatography, Ion Exchange, Copper, Enzyme Stability, Geobacillus, Hydrogen-Ion Concentration, Molecular Weight, Serine Proteases, Substrate Specificity, Surface-Active Agents, Zinc
Kaynak
Applied Biochemistry and Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
193
Sayı
5
