Lilium candidum L.'da In vitro Mikroçoğaltım ve Alkaloidler
Yükleniyor...
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Namık Kemal Üniversitesi, Ziraat Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada in vitro koşullarda mikroçoğaltım yöntemleriyle üretilen Akzambak (Lilium candidum L.) soğanlarında gelişimin gözlendiği dönemden itibaren 3 farklı dönemde (1., 2. ve 3. ay sonunda) soğancıklardan örnek alımı yapılmış ve bu örneklerden alınan soğancıkların ekstraksiyonu yapıldıktan sonra alkaloid (azotlu bileşik) profili GC-MS ile tanımlanmış ve in vivo’da yetişen soğanlarla karşılaştırarak mikroçoğaltım çalışmalarında in vivo koşullara en yakın örnek alım dönemi belirlenmiştir. Çalışmada L. candidum’un soğan pul yaprakları eksplant olarak kullanılmış ve 3 farklı besin ortamından ve 3 farklı dönemde alınan soğancıklarda GC-MS analizi yapılmıştır. Yapılan GC-MS analizi sonucunda soğancıklarda bulunan toplam alkaloid yüzde oranı olarak kontrole (%13.11) en yakın örnek; 2. besin ortamının (0.2 mg/L NAA + 0.4 mg/L TDZ) 3. örnek alım döneminden (%7.42) elde edilmiştir. Belirlenen en yüksek orana sahip alkaloid (1H-Pyrrole, 2,5-dihydro-1-nitroso-) ise yine 2. besin ortamının 3. örnek alım döneminde belirlenmiştir.
In this study, bulblets samples were chosen in produced by in vitro micropropagation method, analyzed by observing the sample from 3 different time periods interferences (i.e. at the end of the first, second and third month). After extraction of these bulblets samples, alkaloid (nitrogen compounds) profile was determined by GC-MS; and was compared with the bulblets grown by in vivo method. Micropropagation analysis, allowed us to find the most suitable sampling period which was close to in vivo condition. In this research, twin scales of L. candidum were used as explant and as a result of regeneration, GC-MS was analyzed in 3 different nutrient media at 3 different sampling periods. The result of GC-MS analysis showed that the total obtained alkaloid percentage is the closed to the control (13.11%) in the third sample (7.42%) in the second nutrient media (0.2 mg/L NAA + 0.4 mg/L TDZ). The highest percentage of alkaloid (1H-Pyrrole, 2,5-dihydro-1-nitroso-) was obtained from the second nutrient medium and the third sample.
In this study, bulblets samples were chosen in produced by in vitro micropropagation method, analyzed by observing the sample from 3 different time periods interferences (i.e. at the end of the first, second and third month). After extraction of these bulblets samples, alkaloid (nitrogen compounds) profile was determined by GC-MS; and was compared with the bulblets grown by in vivo method. Micropropagation analysis, allowed us to find the most suitable sampling period which was close to in vivo condition. In this research, twin scales of L. candidum were used as explant and as a result of regeneration, GC-MS was analyzed in 3 different nutrient media at 3 different sampling periods. The result of GC-MS analysis showed that the total obtained alkaloid percentage is the closed to the control (13.11%) in the third sample (7.42%) in the second nutrient media (0.2 mg/L NAA + 0.4 mg/L TDZ). The highest percentage of alkaloid (1H-Pyrrole, 2,5-dihydro-1-nitroso-) was obtained from the second nutrient medium and the third sample.
Açıklama
Anahtar Kelimeler
Akzambak, mikroçoğaltım, alkaloid, azotlu bileşik, GC-MS, White lily, micropropagation, alkaloid, nitrogen compound
Kaynak
Tekirdağ Ziraat Fakültesi Dergisi
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
13
Sayı
3
