Protective Effect of Alpha Lipoic Acid on Rat Sciatic Nerve Ischemia Reperfusion Damage

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dc.authorid0000-0002-6523-5607
dc.authorid0000-0002-0785-483X
dc.authorid0000-0001-9774-8151
dc.authorid0000-0002-6683-5495
dc.authorscopusid55270334500
dc.authorscopusid6603048599
dc.authorscopusid6603136404
dc.authorscopusid6602371887
dc.authorscopusid37033436700
dc.authorscopusid55765403800
dc.authorscopusid24436194200
dc.authorwosidyagmurca, murat/A-1851-2018
dc.authorwosidMollaoglu, Hakan/C-6987-2013
dc.authorwosidTURAMANLAR, Ozan/ABA-4441-2020
dc.authorwosidTuramanlar, Ozan/AAL-3692-2020
dc.authorwosidYAĞMURCA, Murat/AAH-4496-2019
dc.authorwosidAktas, Cevat/D-8468-2011
dc.contributor.authorTuramanlar, Ozan
dc.contributor.authorÖzen, Oğuz Aslan
dc.contributor.authorSongur, Ahmet
dc.contributor.authorYağmurca, Murat
dc.contributor.authorAkçer, Sezer
dc.contributor.authorMollaoğlu, Hakan
dc.contributor.authorAktaş, Cevat
dc.date.accessioned2022-05-11T14:14:01Z
dc.date.available2022-05-11T14:14:01Z
dc.date.issued2015
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Anatomi Ana Bilim Dalı
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractBackground: Alpha lipoic acid is a potent antioxidant that plays numerous roles in human health. This study examined the effect of ALA on rat sciatic nerve ischemia reperfusion damage. Aims: Protective effect of alpha lipoic acid (ALA) on sciatic nerve following ischemia-reperfusion in rats was investigated by using light microscopy and biochemical methods. Provided that the protective effect of ALA on sciatic nerve is proven, we think the damage to the sciatic nerve that has already occurred or might occur in patients for various reasons maybe prevented or stopped by giving ALA in convenient doses. Study Design: Animal experiment. Methods: Forty-two adult male Sprague-Dawley rats (250-300 grams) were used in this study. Rats were randomly divided into six groups including one control (Group 1), one sham (Group 2), two ischemia-reperfusion (Groups 3 and 4) and two treatment groups (Groups5 and 6). Doses of 60 and 100 mg/kg ALA were given (Group 5 and 6) intra peritoneally twice, 1 and 24 hours before the ischemia to each treatment group. Ischemia was carried out the abdominal aorta starting from the distal part of the renal vein for two hours followed by reperfusion for three hours. In immunohistochemical methods, fibronectin immunoreactivity was analyzed. For biochemical analyses, the tissues were taken in eppendorf microtubes and superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) enzyme activities as well as malondialdehyde (MDA) and nitricoxide (NO) levels were measured. Results: Fibronectin was observed to have increased significantly in the ischemia group; on the other hand, it was observed to have decreased in parallel to the doses in the ALA groups. Biochemical studies showed that SOD and GSHPx declined with ischemia-reperfusion, but the activities of these enzymes were increased in the treatment groups in parallel with the dose. It was found that increased MDA levels with ischemia-reperfusion were decreased in parallel with ALA dose. There were no statistically significant changes in NO. Conclusion: Increased fibronectin observed after ischemia/reperfusion of rat sciatic nerve is reduced after the administration of ALA. This indicates that the function of fibronectin, to reconnect cut nerve segments and regenerate nerves, is more prominent than its function in tissue healing after ischemia. ALA administered before ischemia decreases MDA and increases SOD and GSHPx. We think that ALA may protect against the pathological changes in ischemic nerve and may be used to devise more efficient treatments.
dc.description.sponsorshipAfyon Kocatepe University Research ProjectAfyon Kocatepe University [09.TIP.12]
dc.description.sponsorshipThe authors declared that this study has received financial support from Afyon Kocatepe University Research Project (09.TIP.12).
dc.identifier.doi10.5152/balkanmedj.2015.15619
dc.identifier.endpage202
dc.identifier.issn2146-3123
dc.identifier.issn2146-3131
dc.identifier.issue2en_US
dc.identifier.pmid26167345
dc.identifier.scopus2-s2.0-84929460578
dc.identifier.scopusqualityQ3
dc.identifier.startpage196
dc.identifier.urihttps://doi.org/10.5152/balkanmedj.2015.15619
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5748
dc.identifier.volume32
dc.identifier.wosWOS:000353479200009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzen, Oğuz Aslan
dc.institutionauthorAktaş, Cevat
dc.language.isoen
dc.publisherGalenos Publ House
dc.relation.ispartofBalkan Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAlpha lipoic acid
dc.subjectischemia-reperfusion
dc.subjectsciatic nerve
dc.subjectrat
dc.subjectSuperoxide-Dismutase
dc.subjectLipid-Peroxidation
dc.subjectOxidative Stress
dc.subjectPeripheral-Nerve
dc.subjectNitric-Oxide
dc.subjectInjury
dc.subjectMalondialdehyde
dc.subjectAntioxidant
dc.subjectCatalase
dc.subjectBrain
dc.titleProtective Effect of Alpha Lipoic Acid on Rat Sciatic Nerve Ischemia Reperfusion Damage
dc.typeArticle

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