Are radio-contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

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dc.authorid0000-0001-8171-5929
dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-8084-7855
dc.authorid0000-0001-5590-0637
dc.authorid0000-0002-1224-442X
dc.authorscopusid56674583800
dc.authorscopusid35197435300
dc.authorscopusid7005912992
dc.authorscopusid56769801000
dc.authorscopusid57201362754
dc.authorscopusid56674439000
dc.authorscopusid21644315000
dc.authorwosidAkyuva, Yener/AAV-5706-2020
dc.authorwosidYILMAZ, Ibrahim/H-6199-2019
dc.authorwosidOzbek, Hanefi/O-3472-2019
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.authorwosidKARAARSLAN, Numan/I-6819-2019
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorKaplan, Necati
dc.contributor.authorÇalışkan, Tezcan
dc.contributor.authorAteş, Özkan
dc.date.accessioned2022-05-11T14:12:14Z
dc.date.available2022-05-11T14:12:14Z
dc.date.issued2019
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractIn the literature, there have been no studies showing clear results on how radio-contrast pharmaceuticals would affect intact disc tissue cells. In this context, it was aimed to evaluate the effects of iopromide and gadoxetic acid, frequently used in the discography, on intact lumbar disc tissue in pharmaco-molecular and histopathological level. Primary cell cultures were prepared from the healthy disc tissue of the patients operated in the neurosurgery clinic. Except for the control group, the cultures were incubated with the indicated radio-contrast agents. Cell viability, toxicity and proliferation indices were tested at specific time intervals. The cell viability was quantitatively analysed. It was also visually rechecked under a fluorescence microscope with acridine orange/propidium iodide staining. Simultaneously, cell surface morphology was analysed with an inverted light microscope, while haematoxylin and eosin (H&E) staining methodology was used in the histopathological evaluations. The obtained data were evaluated statistically. Unlike the literature, iopromide or gadoxetic acid did not have any adverse effects on the cell viability, proliferation and toxicity (P < 0.05). Although this study reveals that radio-contrast pharmaceuticals used in the discography, often used in neurosurgical practice, can be safely used, it should be remembered that this study was performed in an in vitro environment.
dc.identifier.doi10.1111/bcpt.13112
dc.identifier.endpage189
dc.identifier.issn1742-7835
dc.identifier.issn1742-7843
dc.identifier.issue2en_US
dc.identifier.pmid30120906
dc.identifier.scopus2-s2.0-85053398131
dc.identifier.scopusqualityQ2
dc.identifier.startpage181
dc.identifier.urihttps://doi.org/10.1111/bcpt.13112
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5461
dc.identifier.volume124
dc.identifier.wosWOS:000456596400006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaraarslan, Numan
dc.institutionauthorŞirin, Duygu Yaşar
dc.institutionauthorÇalışkan, Tezcan
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBasic & Clinical Pharmacology & Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcytotoxicity
dc.subjectdiscography
dc.subjectintact intervertebral disc
dc.subjectprimary cell culture
dc.subjectradio-contrast agents
dc.subjectHigh-Intensity Zone
dc.subjectPain
dc.subjectProliferation
dc.subjectStability
dc.subjectViability
dc.subjectMedia
dc.titleAre radio-contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?
dc.typeArticle

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