Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?

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dc.authorid0000-0001-5590-0637
dc.authorid0000-0002-8084-7855
dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-1224-442X
dc.authorscopusid57201362754
dc.authorscopusid56674583800
dc.authorscopusid35197435300
dc.authorscopusid56769801000
dc.authorscopusid57202017645
dc.authorscopusid56674439000
dc.authorscopusid57202435374
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.authorwosidKARAARSLAN, Numan/I-6819-2019
dc.authorwosidYILMAZ, Ibrahim/H-6199-2019
dc.authorwosidOzbek, Hanefi/O-3472-2019
dc.contributor.authorKaplan, Necati
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorYasar Şirin, Duygu
dc.contributor.authorAkgün, Feride Sinem
dc.contributor.authorÇalışkan, Tezcan
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2022-05-11T14:13:57Z
dc.date.available2022-05-11T14:13:57Z
dc.date.issued2019
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Genetik Ana Bilim Dalı
dc.description.abstractAIM: To investigate the effect of dabigatran, a new oral anticoagulant, on human primary cell cultures isolated from intact intervertebral disc tissue. MATERIAL and METHODS: Cell cultures were prepared from tissues obtained from six cases who had undergone surgery due to spinal trauma. Dabigatran, an active pharmacological agent, was applied to intact annulus fibrosus (AF)/nucleus pulposus (NP) primary cell cultures from the study group. After performing cell viability, toxicity, and proliferation tests on all cultures in the control and study groups, the surface morphologies of the samples were evaluated. Subsequently, chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), and matrix metalloproteinase (MMP)-13 and -19 expressions were measured via a real-time polymerase chain reaction (RT-PCR). Data were analyzed statistically. RESULTS: In the proliferation assays performed on the 20th day of the study, cells in the dabigatran-supplemented group were reported to have lost 46.37% more viability than those in the control group. Expressions of all genes examined except MMP-13 were evaluated in the control group by time, but in contrast to the control group results, COMP and MMP-19 gene expressions decreased in the dabigatran-treated group. No CHAD or MMP-13 expression was noted in these cultures. CONCLUSION: The potential for a systemically applied drug to accumulate in tissue and negatively affect surrounding tissues and microstructures must be emphasized.
dc.identifier.doi10.5137/1019-5149.JTN.24336-18.0
dc.identifier.endpage477
dc.identifier.issn1019-5149
dc.identifier.issue4en_US
dc.identifier.pmid31124572
dc.identifier.scopus2-s2.0-85068917407
dc.identifier.scopusqualityQ3
dc.identifier.startpage470
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.24336-18.0
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5715
dc.identifier.volume29
dc.identifier.wosWOS:000473763100003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaraarslan, Numan
dc.institutionauthorYılmaz, İbrahim
dc.institutionauthorYasar Şirin, Duygu
dc.institutionauthorÇalışkan, Tezcan
dc.institutionauthorÖzbek, Hanefi
dc.language.isoen
dc.publisherTurkish Neurosurgical Soc
dc.relation.ispartofTurkish Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCytotoxicity
dc.subjectDabigatran
dc.subjectDeep vein thrombosis
dc.subjectIntervertebral disc cells
dc.subjectPrimary cell cultures
dc.subjectMatrix
dc.subjectAssociation
dc.subjectProgression
dc.subjectBreast
dc.subjectGrowth
dc.titleAre Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?
dc.typeArticle

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