Role of quercetin in cadmium-induced oxidative stress, testicular damage, and apoptosis in rats
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Science Printers and Publishers Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
OBJECTIVE: The aim of this study was to investigate the role of quercetin on cadmium-induced oxidative stress, testicular damage, and apoptosis in rat testes. STUDY DESIGN: The rats were randomly allotted into 1 of 3 experimental groups: control, cadmium-treated, and cadmium-treated with quercetin; each group contained 10 animals. Control animals received daily injections of the saline vehicle alone. The cadmium-treated group was injected subcutaneously with cadmium chloride (CdCl2) dissolved in saline at a dose of 2 mL/kg/ day for 30 days, resulting in a dosage of 1 mg/kg cadmium. The rats in quercetin-treated groups were given quercetin (15 mg/kg body weight) once a day i.p., starting 2 days prior to the cadmium injection during the study period. All animals were sacrificed and testes tissues were removed for histopathological and biochemical (malondialdehyde [MDA], superoxide dismutase [SOD], glutathione peroxidase [GSH-Px], and serum testosterone levels) investigation. RESULTS: The mean seminiferous tubule diameter, Johnsen's mean testicular biopsy score values, biochemical parameters (MDA, SOD, GSH-Px, and serum testosterone levels), and amount of germ cell apoptosis were significantly decreased in the cadmium-treated groups as compared to the control group. Furthermore, the quercetin-treated animals showed improved histological and biochemical parameters in the cadmium-treated group. CONCLUSION: The present study showed that quercetin treatment protected testes against toxic effects of cadmium. We believe that further preclinical research into the utility of quercetin may indicate its usefulness as a potential treatment for spermatogenesis after testicular injury caused by cadmium-treated rats. © 2016 Science Printers and Publishers, Inc.
Açıklama
Anahtar Kelimeler
3,3',4',5,7-Pentahydroxyflavone, Antioxidants, Apoptosis, Cadmium, Cadmium poisoning, Oxygenradicals, Quercetin, Reactive oxygen species, Testes, Testicles, Testis, Testosterone, cadmium chloride, glutathione peroxidase, malonaldehyde, quercetin, superoxide dismutase, testosterone, quercetin, cadmium, glutathione peroxidase, malonaldehyde, quercetin, superoxide dismutase, animal experiment, animal model, animal tissue, apoptosis, Article, biochemical analysis, controlled study, enzyme blood level, germ cell, histopathology, male, nonhuman, oxidative stress, priority journal, rat, seminiferous tubule, testis biopsy, testis injury, testosterone blood level, animal cell, antioxidant activity, enzyme activity, spermatogenesis, testis injury, testis size, animal, apoptosis, drug effects, human, metabolism, oxidative stress, pathology, testis, Animals, Apoptosis, Cadmium, Glutathione Peroxidase, Humans, Male, Malondialdehyde, Oxidative Stress, Quercetin, Rats, Seminiferous Tubules, Superoxide Dismutase, Testis
Kaynak
Analytical and Quantitative Cytology and Histology
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
38
Sayı
1
