Evaluation of the effects of pregabalin on chondrocyte proliferation and CHAD, HIF-1 alpha, and COL2A1 gene expression

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dc.authorid0000-0001-5590-0637
dc.authorscopusid56769801000
dc.authorscopusid56674583800
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.authorwosidKARAARSLAN, Numan/I-6819-2019
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorKaraarslan, Numan
dc.date.accessioned2022-05-11T14:12:25Z
dc.date.available2022-05-11T14:12:25Z
dc.date.issued2018
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.description.abstractIntroduction: The aim of the present study is to investigate the effects of pregabalin (PGB) on chondrocyte proliferation and collagen type II (COL2A1), hypoxia-inducible factor 1-alpha (HIF-1 alpha), and chondroadherin (CHAD) gene expression in osteoarthritic chondrocytes. Material and methods: Standard primary chondrocyte cultures were prepared using osteochondral tissues that were surgically obtained from 6 patients with gonarthrosis. Cell morphology was evaluated using an inverted microscope, and cell death and proliferation were determined through MTT analysis, which was confirmed by AO/PI staining and statistically evaluated. The expression levels of CHAD, COL2A1, and HIF-1 alpha genes were assessed using gene-specific TaqMan Gene Expression Assays. Results: MTT analyses showed that PGB administration did not have a negative or toxic effect on cell viability and proliferation in cultured chondrocytes (p < 0.001), but in our morphological evaluation extracellular matrix development was observed to be weaker in cultures treated with PGB. After 24 h of treatment, COL2A1, HIF-1 alpha, and CHAD gene expression decreased in the groups to which PGB was applied compared to gene expression before the experiment (at 0 h); at 48 h, CHAD and HIF-1 alpha expression increased to the same level as the control group, but the expression of COL2A1 continued to decrease. Conclusions: Further studies need to be conducted with more participants to prove that there is a negative correlation between extracellular matrix formation and PGB administration. Our preliminary data show that even at low doses and over short-term administration, PGB may affect chondrocyte cells at the gene-expression level.
dc.identifier.doi10.5114/aoms.2018.73134
dc.identifier.endpage1347
dc.identifier.issn1734-1922
dc.identifier.issn1896-9151
dc.identifier.issue6en_US
dc.identifier.pmid30393488
dc.identifier.scopus2-s2.0-85053391282
dc.identifier.scopusqualityQ2
dc.identifier.startpage1340
dc.identifier.urihttps://doi.org/10.5114/aoms.2018.73134
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5549
dc.identifier.volume14
dc.identifier.wosWOS:000448045000014
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorŞirin, Duygu Yaşar
dc.institutionauthorKaraarslan, Numan
dc.language.isoen
dc.publisherTermedia Publishing House Ltd
dc.relation.ispartofArchives of Medical Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCHAD
dc.subjectCOL2A1
dc.subjectHIF-1 alpha
dc.subjectosteoarthritic chondrocytes
dc.subjectpregabalin
dc.subjectBone
dc.subjectGabapentin
dc.subjectDrug
dc.subjectAnticonvulsant
dc.subjectFlurbiprofen
dc.subjectMolecules
dc.subjectSurvival
dc.subjectSubunit
dc.subjectCells
dc.subjectModel
dc.titleEvaluation of the effects of pregabalin on chondrocyte proliferation and CHAD, HIF-1 alpha, and COL2A1 gene expression
dc.typeArticle

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