Transcriptome analysis of the effects of light and dark cycle on hydrogen production metabolism of Rhodobacter capsulatus DSM1710

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dc.authorid0000-0001-5548-1161
dc.authorid0000-0002-6635-3947
dc.authorscopusid35363944400
dc.authorscopusid6505961443
dc.authorscopusid7004598043
dc.authorscopusid7006258822
dc.authorwosidYücel, Meral/AAQ-3548-2021
dc.contributor.authorGürgan, Muazzez
dc.contributor.authorKoku, Harun
dc.contributor.authorEroğlu, İnci
dc.contributor.authorYücel, Meral
dc.date.accessioned2022-05-11T14:28:42Z
dc.date.available2022-05-11T14:28:42Z
dc.date.issued2020
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description4th International Hydrogen Technologies Congress (IHTEC) -- JUN 20-23, 2019 -- Trakya Univ, Edirne, TURKEY
dc.description.abstractPhotosynthetic purple non sulfur bacteria can be utilized in outdoor photobioreactors for sustainable large-scale biohydrogen production. However, the diurnal cycle under natural outdoor conditions affects hydrogen productivity and yield. Here, we investigated the effect of light and dark cycles on the hydrogen production metabolism of Rhodobacter capsulatus DSM1710 using custom-designed Affymetrix Gene Chip technology. R. capsulatus was grown by cyclic illumination on hydrogen producing medium. After three cycles of growth, the comparative transcriptome analysis of the light-period samples versus dark-period samples was carried out. The number of genes showing significant expression change was 914 out of 4052 probe sets in total. The differentially expressed genes were classified into metabolic groups. Genes related to nitrogen metabolism, photosynthesis, electron transport, transporters and central metabolism were up-regulated. In contrast, genes related to protein fate and stress metabolism were down-regulated. These gene expression results provide insight on the influence of the relevant metabolic pathways on hydrogen production under cyclic illumination. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.
dc.description.sponsorshipMiddle East Technical University, Turkey BAP Project [07-02-2013-005]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108T455]
dc.description.sponsorshipThis study was supported by Middle East Technical University, Turkey BAP Project (Project No. 07-02-2013-005) and Scientific and Technological Research Council of Turkey (TUBITAK) (Project No. 108T455) and derived from the PhD Thesis of Muazzez Gurgan. The microarray experiments were carried out at METU Central Lab Molecular Biology and Biotechnology R&D Center. This study was presented in 4th International Hydrogen Technologies Congress, Edirne, Turkey.
dc.identifier.doi10.1016/j.ijhydene.2020.03.108
dc.identifier.endpage34719
dc.identifier.issn0360-3199
dc.identifier.issn1879-3487
dc.identifier.issue60en_US
dc.identifier.scopus2-s2.0-85083322839
dc.identifier.scopusqualityQ1
dc.identifier.startpage34707
dc.identifier.urihttps://doi.org/10.1016/j.ijhydene.2020.03.108
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6915
dc.identifier.volume45
dc.identifier.wosWOS:000609161600014
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorGürgan, Muazzez
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofInternational Journal of Hydrogen Energy
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiological hydrogen production
dc.subjectRhodobacter capsulatus
dc.subjectPurple non sulfur bacteria
dc.subjectDiurnal cycle metabolism
dc.subjectGene expression
dc.subjectMicroarray
dc.subjectBiohydrogen Production
dc.subjectSequential Dark
dc.subjectExpression
dc.subjectIntensity
dc.subjectStrains
dc.subjectAcetate
dc.subjectGene
dc.subjectBiosynthesis
dc.subjectTemperature
dc.subjectPutrescine
dc.titleTranscriptome analysis of the effects of light and dark cycle on hydrogen production metabolism of Rhodobacter capsulatus DSM1710
dc.typeConference Object

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