Ultrasensitive detection of interleukin 1? using 3-phosphonopropionic acid modified FTO surface as an effective platform for disposable biosensor fabrication

Basit Öğe Kaydı
dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:26Z
dc.date.available2022-05-11T14:07:26Z
dc.date.issued2021
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractIn this study, we utilized a carboxyalkylphosphonic acid covered fluorine doped tin oxide (FTO) as an electrode material for fabrication of Interleukin 1? (IL-1?) immunosensor. For this aim, anti-IL-1? antibodies were attached on the 3-phosphonopropionic acid (PHP) modified FTO surface covalently. Electrochemical (electrochemical impedance spectroscopy and cyclic voltammetry) and morphological (scanning electron microscopy and atomic force microscopy) characterizations were performed to monitor the successful fabrication of immunoelectrodes. After incubation of anti-IL-1? antibody immobilized FTO electrodes in IL-1? antigen solutions, increases were seen in impedimetric responses. IL-1? antigen was determined in a linear detection range from 0.02 to 2 pg/mL by EIS. The detection limit of the suggested immunosensor was 6 fg/mL. The applicability of the designed biosensor was tested by using human serum and saliva samples and acceptable results were obtained. In addition, high sensitivity, good specificity, low detection limit made the proposed immunosensor a potential technique for IL-1? antigen determination in routine clinical analysis. © 2020 Elsevier B.V.
dc.identifier.doi10.1016/j.bioelechem.2020.107698
dc.identifier.issn1567-5394
dc.identifier.pmid33254051
dc.identifier.scopus2-s2.0-85096860710
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.bioelechem.2020.107698
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5104
dc.identifier.volume138
dc.identifier.wosWOS:000663533800017
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofBioelectrochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject3-Phosphonopropionic acid
dc.subjectFluorine doped tin oxide
dc.subjectImpedimetric biosensor
dc.subjectInterleukin-1?
dc.subjectAntibodies
dc.subjectAntigens
dc.subjectAtomic force microscopy
dc.subjectElectrochemical impedance spectroscopy
dc.subjectElectrodes
dc.subjectFabrication
dc.subjectImmunosensors
dc.subjectNanocomposites
dc.subjectScanning electron microscopy
dc.subjectTin oxides
dc.subjectClinical analysis
dc.subjectDisposable biosensor
dc.subjectElectrode material
dc.subjectFluorine doped tin oxide
dc.subjectLinear detection ranges
dc.subjectLow detection limit
dc.subjectPotential techniques
dc.subjectUltrasensitive detection
dc.subjectCyclic voltammetry
dc.subject3 phosphonopropionic acid
dc.subjectfluorine
dc.subjectimmobilized antibody
dc.subjectinterleukin 1alpha
dc.subjectpropionic acid derivative
dc.subjecttin oxide
dc.subjectunclassified drug
dc.subject2-phosphonopropionic acid
dc.subjectfluorine
dc.subjectinterleukin 1alpha
dc.subjectphosphonic acid derivative
dc.subjectpropionic acid derivative
dc.subjectstannic oxide
dc.subjecttin derivative
dc.subjectArticle
dc.subjectatomic force microscopy
dc.subjectclinical evaluation
dc.subjectcovalent bond
dc.subjectcyclic voltammetry
dc.subjectelectrochemical analysis
dc.subjecthuman
dc.subjectimpedance spectroscopy
dc.subjectlimit of detection
dc.subjectmeasurement repeatability
dc.subjectreference electrode
dc.subjectreproducibility
dc.subjectsaliva
dc.subjectscanning electron microscopy
dc.subjectsensitivity and specificity
dc.subjectserum
dc.subjectblood
dc.subjectchemistry
dc.subjectdevices
dc.subjectdisposable equipment
dc.subjectgenetic procedures
dc.subjectsurface property
dc.subjectAntibodies, Immobilized
dc.subjectBiosensing Techniques
dc.subjectDisposable Equipment
dc.subjectFluorine
dc.subjectHumans
dc.subjectInterleukin-1alpha
dc.subjectLimit of Detection
dc.subjectOrganophosphonates
dc.subjectPropionates
dc.subjectSaliva
dc.subjectSurface Properties
dc.subjectTin Compounds
dc.titleUltrasensitive detection of interleukin 1? using 3-phosphonopropionic acid modified FTO surface as an effective platform for disposable biosensor fabrication
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
5104.pdf
Boyut:
4.1 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu