Ultrasensitive detection of interleukin 1? using 3-phosphonopropionic acid modified FTO surface as an effective platform for disposable biosensor fabrication
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, we utilized a carboxyalkylphosphonic acid covered fluorine doped tin oxide (FTO) as an electrode material for fabrication of Interleukin 1? (IL-1?) immunosensor. For this aim, anti-IL-1? antibodies were attached on the 3-phosphonopropionic acid (PHP) modified FTO surface covalently. Electrochemical (electrochemical impedance spectroscopy and cyclic voltammetry) and morphological (scanning electron microscopy and atomic force microscopy) characterizations were performed to monitor the successful fabrication of immunoelectrodes. After incubation of anti-IL-1? antibody immobilized FTO electrodes in IL-1? antigen solutions, increases were seen in impedimetric responses. IL-1? antigen was determined in a linear detection range from 0.02 to 2 pg/mL by EIS. The detection limit of the suggested immunosensor was 6 fg/mL. The applicability of the designed biosensor was tested by using human serum and saliva samples and acceptable results were obtained. In addition, high sensitivity, good specificity, low detection limit made the proposed immunosensor a potential technique for IL-1? antigen determination in routine clinical analysis. © 2020 Elsevier B.V.
Açıklama
Anahtar Kelimeler
3-Phosphonopropionic acid, Fluorine doped tin oxide, Impedimetric biosensor, Interleukin-1?, Antibodies, Antigens, Atomic force microscopy, Electrochemical impedance spectroscopy, Electrodes, Fabrication, Immunosensors, Nanocomposites, Scanning electron microscopy, Tin oxides, Clinical analysis, Disposable biosensor, Electrode material, Fluorine doped tin oxide, Linear detection ranges, Low detection limit, Potential techniques, Ultrasensitive detection, Cyclic voltammetry, 3 phosphonopropionic acid, fluorine, immobilized antibody, interleukin 1alpha, propionic acid derivative, tin oxide, unclassified drug, 2-phosphonopropionic acid, fluorine, interleukin 1alpha, phosphonic acid derivative, propionic acid derivative, stannic oxide, tin derivative, Article, atomic force microscopy, clinical evaluation, covalent bond, cyclic voltammetry, electrochemical analysis, human, impedance spectroscopy, limit of detection, measurement repeatability, reference electrode, reproducibility, saliva, scanning electron microscopy, sensitivity and specificity, serum, blood, chemistry, devices, disposable equipment, genetic procedures, surface property, Antibodies, Immobilized, Biosensing Techniques, Disposable Equipment, Fluorine, Humans, Interleukin-1alpha, Limit of Detection, Organophosphonates, Propionates, Saliva, Surface Properties, Tin Compounds
Kaynak
Bioelectrochemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
138
