Effect of growth factors on oocyte maturation and allocations of inner cell mass and trophectoderm cells of cloned bovine embryos

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dc.authorid0000-0002-1140-8058
dc.authorid0000-0002-2789-0635
dc.authorid0000-0003-2991-6322
dc.authorid0000-0002-0754-6116
dc.authorscopusid6603553213
dc.authorscopusid53463162500
dc.authorscopusid23396214300
dc.authorscopusid23767133200
dc.authorscopusid16400954400
dc.authorscopusid55919600100
dc.authorwosidBAĞIŞ/V-3827-2017
dc.authorwosidarat, sezen/ABA-7370-2020
dc.authorwosidÇEVİK, MESUT/AAI-2153-2020
dc.contributor.authorArat, Sezen
dc.contributor.authorCaputcu, Arzu Tas
dc.contributor.authorÇevik, Mesut
dc.contributor.authorAkkoç, Tolga
dc.contributor.authorÇetinkaya, Gaye
dc.contributor.authorBagis, Haydar
dc.date.accessioned2022-05-11T14:45:24Z
dc.date.available2022-05-11T14:45:24Z
dc.date.issued2016
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractThis study was conducted to determine the additive effects of exogenous growth factors during in vitro oocyte maturation (IVM) and the sequential culture of nuclear transfer (NT) embryos. Oocyte maturation and culture of reconstructed embryos derived from bovine granulosa cells were performed in culture medium supplemented with either epidermal growth factor (EGF) alone or a combination of EGF with insulin-like growth factor-I (IGF-I). The maturation rates of oocytes matured in the presence of EGF or the EGF + IGF-I combination were significantly higher than those of oocytes matured in the presence of only fetal calf serum (FCS) (P < 0.05). The developing NT embryos showed no significant differences in fusion, cleavage or blastocyst rates among the culture groups (P > 0.05). IGF-I alone or in combination with EGF in sequential embryo culture medium significantly increased the ratio of inner cell mass (ICM) to total blastocyst cells (P < 0.05). Our results showed that the addition of growth factors to IVM and sequential culture media of cloned bovine embryos increased the ICM without changing the total cell number. These unknown and uncontrolled effects of growth factors can alter the allocation of ICM and trophectoderm cells (TE) in NT embryos. A decrease in TE cell numbers could be a reason for developmental abnormalities in embryos in the cloning system.
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [KAMAG-106G005]
dc.description.sponsorshipWe wish to thanks Dr. O. Yavuz for statistical analysis, Sakir Sekmen, Gazi Turgut and Erman Ates for technical assistance. The manuscript has been edited by native speaker Dr. Anita L. Akkas, who has PhD degree in English Literature and MA degree in Linguistics Engineering and Science. This research was supported by TUBITAK with grant number KAMAG-106G005 and was conducted at TUBITAK-MRC, the Genetic Engineering and Biotechnology Institute in Turkey.
dc.identifier.doi10.1017/S0967199415000519
dc.identifier.endpage562
dc.identifier.issn0967-1994
dc.identifier.issn1469-8730
dc.identifier.issue4en_US
dc.identifier.pmid26444069
dc.identifier.scopus2-s2.0-84943770600
dc.identifier.scopusqualityQ4
dc.identifier.startpage554
dc.identifier.urihttps://doi.org/10.1017/S0967199415000519
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10034
dc.identifier.volume24
dc.identifier.wosWOS:000378857100010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorArat, Sezen
dc.language.isoen
dc.publisherCambridge Univ Press
dc.relation.ispartofZygote
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBovine
dc.subjectCell allocation
dc.subjectCloning
dc.subjectEmbryo culture
dc.subjectGrowth factors
dc.subjectIn-Vitro Fertilization
dc.subjectCulture-Medium
dc.subjectDevelopmental Competence
dc.subjectBlastocyst Development
dc.subjectInsulin
dc.subjectSupplementation
dc.subjectOptimization
dc.subjectApoptosis
dc.subjectReceptor
dc.subjectPorcine
dc.titleEffect of growth factors on oocyte maturation and allocations of inner cell mass and trophectoderm cells of cloned bovine embryos
dc.typeArticle

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