Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2

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dc.authorscopusid14822825900
dc.authorscopusid23488666100
dc.authorscopusid35612683100
dc.authorscopusid56113046700
dc.authorscopusid57115472200
dc.authorscopusid35520123600
dc.authorscopusid23111045300
dc.authorwosidFrazao, Maria/O-4407-2016
dc.contributor.authorYıldırım, Ayşe
dc.contributor.authorGülbol Duran, Gülay
dc.contributor.authorDuran, Nizami
dc.contributor.authorJenedi, Kemal
dc.contributor.authorBolgül, Behiye Sezgin
dc.contributor.authorMiraloğlu, Meral
dc.contributor.authorMuz, Mustafa Necati
dc.date.accessioned2022-05-11T14:42:20Z
dc.date.available2022-05-11T14:42:20Z
dc.date.issued2016
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Parazitoloji Ana Bilim Dalı
dc.description.abstractBackground: Propolis is a bee product widely used in folk medicine and possessing many pharmacological properties. In this study we aimed to investigate: i) the antiviral activities of Hatay propolis samples against HSV-1 and HSV-2 in HEp-2 cell line, and ii) the presence of the synergistic effects of propolis with acyclovir against these viruses. Material/Methods: All experiments were carried out in HEp-2 cell cultures. Proliferation assays were performed in 24-well flat bottom microplates. We inoculated 1x10(5) cells per ml and RPMI 1640 medium with 10% fetal calf serum into each well. Studies to determine cytotoxic effect were performed. To investigate the presence of antiviral activity of propolis samples, different concentrations of propolis (3200, 1600, 800, 400, 200, 100, 75, 50, and 25 mu g/mL) were added into the culture medium. The amplifications of HSV-1 and HSV-2 DNA were performed by realtime PCR method. Acyclovir (Sigma, USA) was chosen as a positive control. Cell morphology was evaluated by scanning electron microscopy (SEM). Results: The replication of HSV-1 and HSV-2 was significantly suppressed in the presence of 25, 50, and 100 mu g/mL of Hatay propolis. We found that propolis began to inhibit HSV-1 replication after 24 h of incubation and propolis activity against HSV-2 was found to start at 48 h following incubation. The activity of propolis against both HSV-1 and HSV-2 was confirmed by a significant decrease in the number of viral copies. Conclusions: We determined that Hatay propolis samples have important antiviral effects compared with acyclovir. In particular, the synergy produced by antiviral activity of propolis and acyclovir combined had a stronger effect against HSV-1 and HSV-2 than acyclovir alone.
dc.identifier.doi10.12659/MSM.897282
dc.identifier.endpage430
dc.identifier.issn1643-3750
dc.identifier.pmid26856414
dc.identifier.scopus2-s2.0-84958038125
dc.identifier.scopusqualityQ2
dc.identifier.startpage422
dc.identifier.urihttps://doi.org/10.12659/MSM.897282
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9336
dc.identifier.volume22
dc.identifier.wosWOS:000369911600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorMuz, Mustafa Necati
dc.language.isoen
dc.publisherInt Scientific Information, Inc
dc.relation.ispartofMedical Science Monitor
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntiviral Agents
dc.subjectDNA Replication
dc.subjectHerpesvirus 1
dc.subjectHuman
dc.subjectHerpesvirus 2, Human
dc.subjectPropolis
dc.subjectAntibacterial Activity
dc.subjectLaboratory Diagnosis
dc.subjectChemical-Composition
dc.subjectViral-Infections
dc.subjectNatural-Products
dc.subjectResistance
dc.subjectAcyclovir
dc.subjectKeratitis
dc.subjectSeroprevalence
dc.subjectEpidemiology
dc.titleAntiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2
dc.typeArticle

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