A Disposable, Highly Sensitive Biosensing System: Determination of Haptoglobin as a Significant Acute Phase Biomarker

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Küçük Resim

Tarih

2016

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Bentham Science Publ Ltd

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

This paper presents a new disposable electrochemical biosensor for the analysis of Haptoglobin (Hp) which shows many important functionalities such as antioxidant capabilities, anti-inflammatory activities and regulation of the immune system. Aminopropyltriethoxysilane (APTES) was self-assembled onto an indium tin oxide (ITO) substrate to fabricate a disposable biosensor. Anti-Hp antibodies were immobilized onto the modified ITO surface covalently. The optimization of the construction parameters and the analytical performance of the biosensor were examined and compared. The methods, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (EIS) were operated for detailed characterization of the different surfaces of the biosensor. Kramers-Kronig Transform was also applied to raw EIS data for checking the verification of obtained experimental EIS data. Moreover, a useful method, single frequency impedance (SFI), was successfully operated to characterize the binding of Hp to the electrode surface modified with anti-Hp. At last, the presented biosensor was applied to the analysis of artificial serum samples, which were spiked with Hp.

Açıklama

Anahtar Kelimeler

Haptoglobin, indium tin oxide, biosensor, acute phase protein, electrochemical impedance spectroscopy, single frequency impedance, Indium-Tin-Oxide, Neuromyelitis-Optica, Impedance Spectroscopy, Porcine Haptoglobin, Immunosorbent-Assay, Cerebrospinal-Fluid, Multiple-Sclerosis, Serum Haptoglobin, Messenger-Rna, Expression

Kaynak

Current Analytical Chemistry

WoS Q Değeri

Q3

Scopus Q Değeri

Q3

Cilt

12

Sayı

1

Künye