Donkey milk kefir induces apoptosis and suppresses proliferation of Ehrlich ascites carcinoma by decreasing iNOS in mice

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dc.authorid0000-0002-3875-2465
dc.authorid0000-0002-7359-6568
dc.authorid0000-0001-9444-3598
dc.authorid0000-0002-1589-9229
dc.authorid0000-0002-0206-6455
dc.authorscopusid55834779800
dc.authorscopusid56694463100
dc.authorscopusid19338014800
dc.authorscopusid57197854869
dc.authorscopusid57213944832
dc.authorscopusid57201555846
dc.authorscopusid6505825773
dc.authorwosidUvez, Ayca/AAH-8851-2019
dc.authorwosidArmutak, Elif/D-2901-2019
dc.authorwosidGurevin, Ebru Gurel/AAC-7921-2020
dc.authorwosidESENER, Osman Behzat Burak/D-1598-2019
dc.authorwosidBALKAN, Burcu Menekşe/AAP-3009-2020
dc.contributor.authorEsener, Osman Behzat Burak
dc.contributor.authorBalkan, B. M.
dc.contributor.authorArmutak, Elif İlkay İkitimur
dc.contributor.authorUvez, A.
dc.contributor.authorYıldız, Günay
dc.contributor.authorHafizoglu, M.
dc.contributor.authorGurel-Gürevin, E.
dc.contributor.authorYılmazer, Nedim
dc.date.accessioned2022-05-11T14:28:39Z
dc.date.available2022-05-11T14:28:39Z
dc.date.issued2018
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractDonkey milk and donkey milk kefir exhibit antiproliferative, antimutagenic and antibacterial effects. We investigated the effects of donkey milk and donkey milk kefir on oxidative stress, apoptosis and proliferation in Ehrlich ascites carcinoma (EAC) in mice. Thirty-four adult male Swiss albino mice were divided into four groups as follows: group 1, administered 0.5ml water; group 2, administered 0.5ml water + EAC cells; group 3, administered 0.5ml donkey milk + EAC cells; group 4, administered 0.5ml donkey milk kefir + EAC cells. We introduced 2.5 x 10(6) EAC cells into each animal by subcutaneous injection. Tap water, donkey milk and donkey milk kefir were administered by gavage for 10days. Animals were sacrificed on day 11. After measuring the short and long diameters of the tumors, tissues were processed for histology. To determine oxidative stress, cell death and proliferation iNOS and eNOS, active caspase-3 and proliferating cell nuclear antigen were assessed using immunohistochemistry. A TUNEL assay also was used to detect apoptosis. Tumor volume decreased in the donkey milk kefir group compared to the control and donkey milk groups. Tumor volume increased in the donkey milk group compared to the control group. Proliferating cell nuclear antigen levels were higher in the donkey milk kefir group compared to the control and donkey milk groups. The number of apoptotic cells was less in the donkey milk group, compared to the control, whereas it was highest in the donkey milk kefir group. Donkey milk administration increased eNOS levels and decreased iNOS levels, compared to the control group. In the donkey milk kefir group, iNOS levels were significantly lower than those of the control and donkey milk groups, while eNOS levels were similar to the control group. Donkey milk kefir induced apoptosis, suppressed proliferation and decreased co-expression of iNOS and eNOS. Donkey milk promoted development of the tumors. Therefore, donkey milk kefir appears to be more beneficial for treating breast cancer than donkey milk.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul UniversityIstanbul University [BEK-2017-25137]
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Coordination Unit of Istanbul University [Project number: BEK-2017-25137].
dc.identifier.doi10.1080/10520295.2018.1448112
dc.identifier.endpage431
dc.identifier.issn1052-0295
dc.identifier.issn1473-7760
dc.identifier.issue6en_US
dc.identifier.pmid29642726
dc.identifier.scopus2-s2.0-85045273967
dc.identifier.scopusqualityQ2
dc.identifier.startpage424
dc.identifier.urihttps://doi.org/10.1080/10520295.2018.1448112
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6898
dc.identifier.volume93
dc.identifier.wosWOS:000447625300004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorYılmazer, Nadim
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofBiotechnic & Histochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectapoptosis
dc.subjectcancer
dc.subjectdonkey
dc.subjectkefir
dc.subjectmilk
dc.subjectmouse
dc.subjectNitric-Oxide Synthase
dc.subjectHuman Breast-Cancer
dc.subjectAdenocarcinoma Cells
dc.subjectDairy-Products
dc.subjectRisk
dc.subjectExpression
dc.subjectEstrogen
dc.subjectConsumption
dc.subjectGrowth
dc.subjectGrade
dc.titleDonkey milk kefir induces apoptosis and suppresses proliferation of Ehrlich ascites carcinoma by decreasing iNOS in mice
dc.typeArticle

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