A comparative study of short chain and long chain mercapto acids used in biosensor fabrication: A VEGF-R1-based immunosensor as a model system

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dc.authorscopusid56465916100
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorBahadır, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:12:19Z
dc.date.available2022-05-11T14:12:19Z
dc.date.issued2016
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractA novel impedimetric biosensor utilizing a biological receptor, vascular endothelial growth factor receptor-1 (VEGF-R1), was developed for the determination of vascular endothelial growth factor (VEGF). VEGF-R1 was covalently immobilized by coupling with 11-mercaptoundecanoic acid, which formed a self-assembled monolayer on gold electrodes. Cyclic voltammetry and electrochemical impedance spectroscopy techniques were employed to characterize the immobilization process and to detect VEGF. To successfully construct the biosensor current, the experimental parameters were optimized. A Kramers-Kronig transform was performed on the experimental impedance data. The results obtained provided a linear response range from 1 to 6 ng/mL human VEGF. The applicability of the biosensor developed to determine VEGF in a spiked artificial human serum sample was tested. The important parameters related with the biosensors fabricated by using two different mercapto acids were compared in terms of the self-assembly processes, the activation conditions of -COOH ends, linear ranges obtained for VEGF, repeatabilities and reproducibilities, and cleaning procedures. The results of this study revealed that the length of the mercapto acids used for biosensor fabrication considerably affected the analytical performance and the practicability of the preparation of the biosensor.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipThis work was financially supported by TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678).
dc.identifier.doi10.3109/21691401.2014.962743
dc.identifier.endpage470
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue2en_US
dc.identifier.pmid25289447
dc.identifier.scopus2-s2.0-84964335246
dc.identifier.scopusqualityQ1
dc.identifier.startpage462
dc.identifier.urihttps://doi.org/10.3109/21691401.2014.962743
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5496
dc.identifier.volume44
dc.identifier.wosWOS:000370635000005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBahadır, Elif Burcu
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofArtificial Cells Nanomedicine and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject11-mercaptoundecanoic acid
dc.subjectVEGF
dc.subjectVEGF-R1
dc.subject3-mercaptopropionic acid
dc.subjectelectrochemical impedance spectroscopy
dc.subjectbiosensor
dc.subjectEndothelial Growth-Factor
dc.subjectVascular-Permeability Factor
dc.subjectElectrochemical Impedance Spectroscopy
dc.subjectVegf Receptor-1
dc.subjectX-Rays
dc.subjectGold
dc.subjectMonolayers
dc.subjectWater
dc.subjectCarbodiimides
dc.subjectAngiogenesis
dc.titleA comparative study of short chain and long chain mercapto acids used in biosensor fabrication: A VEGF-R1-based immunosensor as a model system
dc.typeArticle

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