The association between different molecular weights of hyaluronic acid and CHAD, HIF-1 alpha, COL2A1 expression in chondrocyte cultures

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dc.authorid0000-0001-8171-5929
dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-8084-7855
dc.authorid0000-0001-5590-0637
dc.authorscopusid56769801000
dc.authorscopusid57201362754
dc.authorscopusid35197435300
dc.authorscopusid56674583800
dc.authorscopusid7005912992
dc.authorscopusid56033997900
dc.authorscopusid57194611714
dc.authorwosidAkkaya, Nuray/AAP-9977-2020
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.authorwosidözbek, hanefi/P-3492-2015
dc.authorwosidAkyuva, Yener/AAV-5706-2020
dc.authorwosidYILMAZ, Ibrahim/H-6199-2019
dc.authorwosidMahirogullari, Mahir/AAA-4742-2020
dc.authorwosidOzbek, Hanefi/O-3472-2019
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorKaplan, Necati
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorAkyuva, Yener
dc.contributor.authorMahiroğulları, Mahir
dc.date.accessioned2022-05-11T14:12:14Z
dc.date.available2022-05-11T14:12:14Z
dc.date.issued2018
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractThe aim of the present study was to investigate the effects of three different formulations of hyaluronic acid (HA): Low molecular weight (MW) Sinovial One((R)), medium MW Viscoplus((R)) and high MW Durolane((R)), on chondrocyte proliferation and collagen type II (COL2A1), hypoxia-inducible factor 1 alpha (HIF-1 alpha) and chondroadherin (CHAD) expression in primary chondrocyte cultures. Standard primary chondrocyte cultures were established from osteochondral tissues surgically obtained from 6 patients with gonarthrosis. Cell morphology was evaluated using an inverted light microscope; cell proliferation was determined with a MTT assay and confirmed with acridine orange/propidium iodide staining. Levels of CHAD, COL2A1 and HIF-1 alpha expression were assessed using specific TaqMan gene expression assays. The results demonstrated the positive effect of HA treatment on cell proliferation, which was independent from the MW. COL2A1 expression increased in the medium and high MW HA treated groups. It was observed that HIF-1 alpha expression increased in the high MW treated group alone. CHAD expression increased only in the medium MW HA treated group. Evaluation of gene expression revealed that levels of expression increased as the duration of HA application increased, in the medium and high MW HA treated groups. In terms of increased viability and proliferation, a longer duration of HA application was more effective. Taken together, it may be concluded that the administration of medium and high MW HA may be a successful way of treating diseases affecting chondrocytes in a clinical setting.
dc.identifier.doi10.3892/etm.2018.5943
dc.identifier.endpage4212
dc.identifier.issn1792-0981
dc.identifier.issn1792-1015
dc.identifier.issue5en_US
dc.identifier.pmid29849772
dc.identifier.scopus2-s2.0-85044444457
dc.identifier.scopusqualityN/A
dc.identifier.startpage4205
dc.identifier.urihttps://doi.org/10.3892/etm.2018.5943
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5460
dc.identifier.volume15
dc.identifier.wosWOS:000431850300018
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaraarslan, Numan
dc.institutionauthorŞirin, Duygu Yaşar
dc.language.isoen
dc.publisherSpandidos Publ Ltd
dc.relation.ispartofExperimental and Therapeutic Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectchondroadherin
dc.subjectcollagen type II
dc.subjecthypoxia-inducible factor 1 alpha
dc.subjecthyaluronic acid
dc.subjectchondrocytes
dc.subjectKnee Osteoarthritis
dc.subjectQuantitative Pcr
dc.subjectInjections
dc.titleThe association between different molecular weights of hyaluronic acid and CHAD, HIF-1 alpha, COL2A1 expression in chondrocyte cultures
dc.typeArticle

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