Combining dimethyl sulphoxide (DMSO) with different cryoprotectants ensures better cartilage cell cryopreservation

Basit Öğe Kaydı
dc.authorscopusid57201433800
dc.authorscopusid6603553213
dc.contributor.authorTüten Sevim, Emel
dc.contributor.authorArat, S.
dc.date.accessioned2022-05-11T14:45:30Z
dc.date.available2022-05-11T14:45:30Z
dc.date.issued2021
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractBACKGROUND: DMSO is a cryoprotective agent (CPA) that is widely used in the cryopreservation of cells. However, evidence suggests that it is more effective when combined with other CPAs. OBJECTIVE: To investigated the effect of combining serum and balanced solutions with DMSO for cryopreservation. MATERIALS AND METHODS: Cartilage cells cultured with Dulbecco's Modified Eagle Medium (DMEM) medium were taken from the ears of adult cattle. Then, these cells were frozen by supplementation with different concentrations of fetal bovine serum (FBS) and different balanced solutions with DMSO. RESULTS: The highest cell viability was obtained using freezing solutions containing 10% DMSO and 40% serum, in dextran 40 or dextrose solution or DMEM. CONCLUSION: Our results indicated that serum concentration is important for cell viability and that supplementing DMSO with dextran or dextrose or DMEM benefits the cryopreservation of bovine cartilage cells.
dc.identifier.endpage226
dc.identifier.issn0143-2044
dc.identifier.issue4en_US
dc.identifier.pmid35363841
dc.identifier.scopus2-s2.0-85127508605
dc.identifier.scopusqualityQ3
dc.identifier.startpage220
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10061
dc.identifier.volume42
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTüten Sevim, Emel
dc.institutionauthorArat, S.
dc.language.isoen
dc.publisherNLM (Medline)
dc.relation.ispartofCryo letters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleCombining dimethyl sulphoxide (DMSO) with different cryoprotectants ensures better cartilage cell cryopreservation
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Ziraat Fakültesi Koleksiyonu