Graphene oxide based electrochemical label free immunosensor for rapid and highly sensitive determination of tumor marker HSP70

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dc.authorid0000-0002-5123-5972
dc.authorscopusid56106034900
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidÖzcan, Burcu/AAY-1451-2021
dc.contributor.authorÖzcan, Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:30:55Z
dc.date.available2022-05-11T14:30:55Z
dc.date.issued2016
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractIn this study, it is aimed to design a label free immunosensor for determination of HSP70 (heat shock protein 70). Glassy carbon electrode was used as a working electrode. Graphene oxide was covered on the working electrode surface. AntiHSP70 as a biorecognition element of the biosensor was covalently immobilized onto the graphene oxide layer by using EDC/NHS chemistry. The immobilization of antiHSP70 and binding of HSP70 protein onto the electrode surface were monitored by cyclic voltammetry and electrochemical impedance spectroscopy. Single frequency technique was also utilized to monitor binding characterization of HSP70 and antiHSP70. Surface morphology was defined by using scanning electron microscopy. All important fabrication steps of the biosensor were optimized to prepare an ultrasensitive biosensor. Under optimum conditions, analytical studies such as linearity, repeatability, and reproducibility were also experienced. A linear detection range of HSP70 was determined between 12 and 144 fg/mL. Moreover, Kramer's Kronig transform was applied on impedance data. Finally, the biosensor was applied with real human blood serum samples and hopeful results were obtained. (C) 2016 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipThis work was funded by TUBITAK (The Scientific and Technological Research Council of Turkey, (Project number: 113 Z 678), this assistance is greatly acknowledged.
dc.identifier.doi10.1016/j.talanta.2016.07.039
dc.identifier.endpage374
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid27591626
dc.identifier.scopus2-s2.0-84989947777
dc.identifier.scopusqualityQ1
dc.identifier.startpage367
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2016.07.039
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7227
dc.identifier.volume160
dc.identifier.wosWOS:000383524400047
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzcan, Burcu
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGraphene oxide
dc.subjectHSP70
dc.subjectCyclic voltammetry (CV)
dc.subjectElectrochemical impedance spectroscopy (EIS)
dc.subjectLabel free immunosensor
dc.subjectHeat-Shock Proteins
dc.subjectMolecular Chaperones
dc.subjectStress-Response
dc.subjectCancer
dc.subjectHeat-Shock-Protein-70
dc.subjectBiosensor
dc.subjectElectrodes
dc.subjectPhysiology
dc.subjectChemistry
dc.subjectBiomarker
dc.titleGraphene oxide based electrochemical label free immunosensor for rapid and highly sensitive determination of tumor marker HSP70
dc.typeArticle

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