The protective effects of endothelin-A receptor antagonist BQ-123 in pentylenetetrazole-induced seizure in rats
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Sage Publications Ltd
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Endothelin-1 has been shown to increase neuronal activity and glutaminergic synaptic transmission by endothelin-A receptors (ETAR) in the nucleus tractus solitarius neurons that play an important role in epileptic seizures. Therefore, BQ-I23 as an ETAR antagonist might attenuate neuronal excitability and glutaminergic synaptic transmission. The main purpose of the present study is to investigate the protective effect of acute BQ-123 treatment against pentylenetetrazole (PTZ)-induced tonic-clonic seizures. Wistar albino rats were divided into three groups: control, PTZ, and PTZ + BQ-123 groups. BQ-123 (3 mg/kg, intravenously) was administered for 15 min before injecting with PTZ (50 mg/kg, intraperitoneally). We determined a delay resulting from BQ-123 in duration of the seizure onset. Number of rats with major seizure also decreased according to scoring with video camera in PTZ + BQ-123 group. In BQ-123-treated group, there were eight rats without a major seizure, but only one rat had a delayed major seizure. The brain tissue glutathione peroxidase activity was significantly decreased in the PTZ and PTZ BQ-123 groups. According to the results of the control group, there was a significant increase in the protein carbonyl levels of the PTZ group and a significant increase in the nitric oxide levels of the PTZ + BQ-123 group. Histological examination showed an increase in the number of neuronal hyperchromatic nucleus especially in hippocampal gyrus dentatus region of BQ-123-treated group. We concluded that BQ-123 impeded the formation and spread of seizure to a great degree. The beneficial effects of BQ-I23 were comparatively supported with biochemical parameters and histological examinations.
Description
Keywords
Pentylenetetrazole, seizure, BQ-123, endothelin receptor antagonist, Induced Epileptiform Activity, Ischemia-Reperfusion Injury, Acid Phenethyl Ester, Alpha-Tocopherol, Nitric-Oxide, Superoxide-Dismutase, Cell-Proliferation, Oxidative Stress, Dentate Gyrus, Brain
Journal or Series
Human & Experimental Toxicology
WoS Q Value
Q3
Scopus Q Value
Q2
Volume
33
Issue
10
