Therapeutic effect of bovine amniotic fluid in murine dry eye model

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dc.authorid0000-0002-6243-4221
dc.authorscopusid55316940600
dc.authorscopusid7006243697
dc.authorscopusid6603312224
dc.authorscopusid6602225386
dc.authorscopusid27367627900
dc.authorwosidAslan, Abdullah/C-9523-2016
dc.contributor.authorKulualp, Kadri
dc.contributor.authorKılıç, Servet
dc.contributor.authorEroksuz, Yesari
dc.contributor.authorEroksuz, Hatice
dc.contributor.authorAslan, Abdullah
dc.date.accessioned2022-05-11T14:42:14Z
dc.date.available2022-05-11T14:42:14Z
dc.date.issued2020
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü, Cerrahi Ana Bilim Dalı
dc.description.abstractThis study investigated the therapeutic effect of bovine amniotic fluid (BAF) in dry eye (DE) model in 60 female BALB/c mice divided equally into 6 groups. Control group (CG) received 5 mu L formal saline and experimental groups 0.2% benzalkonium chloride in both eyes twice a day during 14 days. From 15 to 30 days while CG and DE group only was administered saline, other groups called BAF20, BAF35, BAF50 and BAF100 received 5 mu L 20, 35, 50 and 100% BAF three times a day, respectively. On day 15, in all experimental groups tear production decreased, tear break-up time shortened, corneal fluorescein staining score increased compared to baseline; on day 30, for these parameters the most effective BAF concentrations were 35%, 50% and 100%. According to western blot analysis the lowest levels were obtained in CG and BAF35 groups for tumor necrosis factora (TNF-alpha), vascular endothelial growth factor (VEGF) and cytokeratin 10 (K10); CG for adiponectin receptor-1 (AdipoR1); BAF35 and CG for adiponectin receptor-2 (AdipoR2) (P < 0.05). In the immunohistochemical analysis the lowest levels were in CG, BAF35, BAF50 and BAF100 groups for TNF-alpha, VEGF and K10; CG, BAF35 and BAF50 groups for AdipoR1; CG, BAF20, BAF35 and BAF50 groups for AdipoR2 (P < 0.05). TUNEL method revealed a lower apoptotic cell score in all BAF groups (P > 0.05). In conclusion; moderate to high concentrations of BAF have the more beneficial effects on DE at molecular and clinical signs and it can be used for the treatment.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [116O927]
dc.description.sponsorshipThis study was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK). (Project number: 116O927). The data of this study were presented as an oral presentation at the 2nd International Veterinary Surgery Congress of Turkey.
dc.identifier.doi10.14943/jjvr.68.3.171
dc.identifier.endpage186
dc.identifier.issn0047-1917
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85092058969
dc.identifier.scopusqualityQ3
dc.identifier.startpage171
dc.identifier.urihttps://doi.org/10.14943/jjvr.68.3.171
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9292
dc.identifier.volume68
dc.identifier.wosWOS:000575870500004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKılıç, Servet
dc.language.isoen
dc.publisherHokkaido Univ
dc.relation.ispartofJapanese Journal of Veterinary Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbovine amniotic fluid
dc.subjectdry eye
dc.subjectinflammation
dc.subjectmice
dc.subjecttreatment
dc.subjectOcular Surface Changes
dc.subjectBenzalkonium Chloride
dc.subjectMouse Model
dc.subjectEpithelial-Cells
dc.subjectTear Production
dc.subjectRabbit
dc.subjectAgents
dc.subjectInflammation
dc.subjectAdiponectin
dc.subjectEfficacy
dc.titleTherapeutic effect of bovine amniotic fluid in murine dry eye model
dc.typeArticle

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