Co-circulation of West Nile virus and distinct insect-specific flaviviruses in Turkey

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dc.authorid0000-0001-5008-9443
dc.authorid0000-0001-5422-1982
dc.authorid0000-0002-2583-6264
dc.authorid0000-0002-5673-4180
dc.authorid0000-0003-0957-3377
dc.authorid0000-0002-9711-4113
dc.authorid0000-0001-8185-3176
dc.authorscopusid6507415764
dc.authorscopusid23566817100
dc.authorscopusid55258915300
dc.authorscopusid37114339500
dc.authorscopusid57191475147
dc.authorscopusid15769247000
dc.authorscopusid55881292900
dc.authorwosidOzkul, Aykut/A-1973-2016
dc.authorwosidLinton, Yvonne/AAG-2353-2019
dc.authorwosidkasap, ozge erisoz/J-3896-2013
dc.authorwosidErgunay, Koray/I-8368-2013
dc.authorwosidGunay, Filiz/AAC-7874-2020
dc.authorwosidKASAP, OZGE ERISOZ/AAH-3050-2020
dc.authorwosidLinton, Yvonne-Marie/AAW-3992-2021
dc.contributor.authorErgünay, Koray
dc.contributor.authorLitzba, Nadine
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorGunay, Filiz
dc.contributor.authorSarıkaya, Yasemen
dc.contributor.authorKar, Sırrı
dc.contributor.authorLinton, Yvonne-Marie
dc.date.accessioned2022-05-11T14:28:36Z
dc.date.available2022-05-11T14:28:36Z
dc.date.issued2017
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractBackground: Active vector surveillance provides an efficient tool for monitoring the presence or spread of emerging or re-emerging vector-borne viruses. This study was undertaken to investigate the circulation of flaviviruses. Mosquitoes were collected from 58 locations in 10 provinces across the Aegean, Thrace and Mediterranean Anatolian regions of Turkey in 2014 and 2015. Following morphological identification, mosquitoes were pooled and screened by nested and real-time PCR assays. Detected viruses were further characterised by sequencing. Positive pools were inoculated onto cell lines for virus isolation. Next generation sequencing was employed for genomic characterisation of the isolates. Results: A total of 12,711 mosquito specimens representing 15 species were screened in 594 pools. Eleven pools (2%) were reactive in the virus screening assays. Sequencing revealed West Nile virus (WNV) in one Culex pipiens (s. l.) pool from Thrace. WNV sequence corresponded to lineage one clade 1a but clustered distinctly from the Turkish prototype isolate. In 10 pools, insect-specific flaviviruses were characterised as Culex theileri flavivirus in 5 pools of Culex theileri and one pool of Cx. pipiens (s. l.), Ochlerotatus caspius flavivirus in two pools of Aedes (Ochlerotatus) caspius, Flavivirus AV-2011 in one pool of Culiseta annulata, and an undetermined flavivirus in one pool of Uranotaenia unguiculata from the Aegean and Thrace regions. DNA forms or integration of the detected insect-specific flaviviruses were not observed. A virus strain, tentatively named as Ochlerotatus caspius flavivirus Turkey, was isolated from an Ae. caspius pool in C6/36 cells. The viral genome comprised 10,370 nucleotides with a putative polyprotein of 3,385 amino acids that follows the canonical flavivirus polyprotein organisation. Sequence comparisons and phylogenetic analyses revealed the close relationship of this strain with Ochlerotatus caspius flavivirus from Portugal and Hanko virus from Finland. Several conserved structural and amino acid motifs were identified. Conclusions: We identified WNV and several distinct insect-specific flaviviruses during an extensive biosurveillance study of mosquitoes in various regions of Turkey in 2014 and 2015. Ongoing circulation of WNV is revealed, with an unprecedented genetic diversity. A probable replicating form of an insect flavivirus identified only in DNA form was detected.
dc.description.sponsorshipU.S. Armed Forces Health Surveillance Board Global Emerging Infections Surveillance and Response System (AFHSB-GEIS) research; Walter Reed Army Institute of Research; Smithsonian InstitutionSmithsonian Institution; Georg Forster Research Fellowship (HERMES); Alexander von Humboldt Foundation, GermanyAlexander von Humboldt Foundation
dc.description.sponsorshipA U.S. Armed Forces Health Surveillance Board Global Emerging Infections Surveillance and Response System (AFHSB-GEIS) research award (to YML) supported this study. This research was performed in part under a Memorandum of Understanding between the Walter Reed Army Institute of Research and the Smithsonian Institution, with institutional support provided by both organisations. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The material to be published reflects the views of the authors and should not be construed to represent those of the United States Department of the Army or the United States Department of Defense. KE was a 2015 recipient of the Georg Forster Research Fellowship (HERMES) for Experienced Researchers, of the Alexander von Humboldt Foundation, Germany.
dc.identifier.doi10.1186/s13071-017-2087-7
dc.identifier.issn1756-3305
dc.identifier.pmid28320443
dc.identifier.scopus2-s2.0-85015715309
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/s13071-017-2087-7
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6885
dc.identifier.volume10
dc.identifier.wosWOS:000396900000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKar, Sırrı
dc.language.isoen
dc.publisherBmc
dc.relation.ispartofParasites & Vectors
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectWest Nile virus
dc.subjectFlavivirus
dc.subjectInsect-specific
dc.subjectBiosurveillance
dc.subjectMosquito
dc.subjectTurkey
dc.subjectField-Collected Mosquitos
dc.subjectSurveillance
dc.subjectAlignment
dc.subjectSequence
dc.titleCo-circulation of West Nile virus and distinct insect-specific flaviviruses in Turkey
dc.typeArticle

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