Detection of xanthomonas axonopodis pv. vesicatoria in naturally infected pepper seeds in turkey

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dc.authorscopusid55948751100
dc.authorscopusid56429421300
dc.contributor.authorMırık, Mustafa
dc.contributor.authorAysan, Yeşim
dc.date.accessioned2022-05-11T14:43:27Z
dc.date.available2022-05-11T14:43:27Z
dc.date.issued2009
dc.departmentFakülteler, Ziraat Fakültesi, Bitki Koruma Bölümü
dc.description.abstractBacterial spot, caused by Xanthomonas axonopodis pv. vesicatoria, is one of the most important diseases of pepper in Turkey, where the disease has been epidemic in the last six years in the eastern Mediterranean region. The incidence and importance of natural infection in pepper seeds produced in the region was investigated in the present study. A total of 29 seed samples were tested by immunofluorescence assay using a commercial specific antibody, semi-selective isolation on medium Tween B, and a seedling screening tests, where plantlets were examined for typical spot symptoms on cotyledons 7-14 days after emergence. Pathogen populations were found to be in the range of 5×101-5×104 cells/g seed when using semi-selective medium Tween B. The diseased seeds as determined in the seedling screening ranged from 7 to 36%; these figures agreed with numbers found in the Tween B medium test. The strains isolated on Tween B were identified by PCR using primer designed on the hrp gene specific for Xanthomonas spp., and pathogenicity on pepper plants. Twenty-one out of 29 seed samples were found to be contaminated by Xanthomonas axonopodis pv. vesicatoria. On the basis of these findings, infested seeds must be seen as a major source of inoculum for this disease in the region.
dc.identifier.endpage436
dc.identifier.issn1125-4653
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-77749313226
dc.identifier.scopusqualityQ2
dc.identifier.startpage433
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9606
dc.identifier.volume91
dc.indekslendigikaynakScopus
dc.institutionauthorMırık, Mustafa
dc.language.isoen
dc.relation.ispartofJournal of Plant Pathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEpidemiology
dc.subjectIFAS
dc.subjectPCR
dc.subjectPepper
dc.subjectSeed
dc.subjectXanthomonas axonopodis pv. vesicatoria
dc.subjectBacteria (microorganisms)
dc.subjectXanthomonas
dc.subjectXanthomonas axonopodis
dc.subjectXanthomonas axonopodis pv. vesicatoria
dc.titleDetection of xanthomonas axonopodis pv. vesicatoria in naturally infected pepper seeds in turkey
dc.typeArticle

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