Assessing the negative impact of phenyl alkanoic acid derivative, a frequently prescribed drug for the suppression of pain and inflammation, on the differentiation and proliferation of chondrocytes

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dc.authorid0000-0003-3307-085X
dc.authorid0000-0003-2003-6337
dc.authorid0000-0001-6534-9201
dc.authorscopusid56117583100
dc.authorscopusid35197435300
dc.authorscopusid56258081800
dc.authorscopusid56769801000
dc.authorscopusid23666416400
dc.authorscopusid23471057500
dc.authorscopusid56891784100
dc.authorwosidUgras, Ali/AAR-1497-2021
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.authorwosidYILMAZ, Ibrahim/H-6199-2019
dc.authorwosidCiftci, Zafer/AAW-9972-2021
dc.authorwosidUgras, Ali Akin/AAR-2402-2020
dc.authorwosidMahirogullari, Mahir/AAA-4742-2020
dc.contributor.authorGümüştaş, Seyit Ali
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorİşyar, Mehmet
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorBatmaz, Ahmet Güray
dc.contributor.authorUğraş, Ali Akın
dc.contributor.authorMahiroğulları, Mahir
dc.contributor.authorÇiftçi, Zafer
dc.date.accessioned2022-05-11T14:12:28Z
dc.date.available2022-05-11T14:12:28Z
dc.date.issued2016
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Kulak Burun ve Boğaz Hastalıkları Ana Bilim Dalı
dc.description.abstractBackground: Non-steroidal anti-inflammatory drugs (NSAIDs) are frequently prescribed to relieve pain and inflammation. These NSAIDs have also analgesic effects and can be administered via oral, injectable, and topical routes. During inflammation, a number of synovial mediators and cytokines are released which decrease the pH level of the synovial fluid. Administration of acidic NSAIDs further decreases the pH levels and hence contributes to the destruction of the cartilage. To our knowledge, no cellular-based study regarding the chondrotoxicity of phenyl alkanoic acid derivatives on NSAIDs was conducted before. Thus, the aim of this pioneering study was to examine the effect of frequently prescribed NSAIDs, a phenyl alkanoic acid derivative, flurbiprofen, on the proliferation and differentiation of human primer chondrocyte cultures in vitro. Methods: Primer chondrocyte cultures were prepared from osteochondral tissue obtained during surgery for gonarthrosis. Samples not exposed to the pharmacological agent were used as the control group. The samples were treated with 1, 10, 100, 250, 500, or 1000 mu M of the agent for 24, 48, and 72 h. The cell viability, toxicity, and proliferation were assessed with MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) analysis and prechondrocytic precursor stage-specific embryonic antigen-1 (SSEA-1) expression using a commercial ELISA kit spectrophotometrically. The surface morphology of the samples in each group was compared using an inverted light microscope and an environmental scanning electron microscope (ESEM). An analysis of variance was used to compare between-group differences. Tukey's honest significant difference (HSD) method (95 % confidence interval) was used to evaluate the differences and significance in averages. The alpha significance value was considered < 0.01. Results: Statistically significant cytotoxicity was observed in the treatment groups. NSAID had a significant negative effect on the proliferation and differentiation of chondrocytes as compared to the control group (p < 0.01). Conclusion: Before administering phenyl alkanoic acid derivatives in the clinical setting, their role in suppressing the proliferation and differentiation of chondrocytes should be taken into account. Thus, caution should be given when prescribing these drugs.
dc.identifier.doi10.1186/s13018-016-0406-x
dc.identifier.issn1749-799X
dc.identifier.pmid27363505
dc.identifier.scopus2-s2.0-84976439895
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/s13018-016-0406-x
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5571
dc.identifier.volume11
dc.identifier.wosWOS:000379205000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorŞirin, Duygu Yaşar
dc.institutionauthorÇiftçi, Zafer
dc.language.isoen
dc.publisherBmc
dc.relation.ispartofJournal of Orthopaedic Surgery and Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPhenyl alkanoic acid
dc.subjectChondrotoxicity
dc.subjectProliferation
dc.subjectStage-specific
dc.subjectEmbryonic
dc.subjectAntigen-1
dc.subjectSynovial-Fluid
dc.subjectMetaanalysis
dc.subjectViability
dc.subjectPlaster
dc.subjectNsaids
dc.titleAssessing the negative impact of phenyl alkanoic acid derivative, a frequently prescribed drug for the suppression of pain and inflammation, on the differentiation and proliferation of chondrocytes
dc.typeArticle

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