Genetic relationship and nuclear dna content variation in Tef [Eragrostis tef (Zucc.) Trotter] accessions

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dc.authorid0000-0002-7504-2892
dc.authorscopusid57216935077
dc.authorscopusid6602664701
dc.authorscopusid57216937593
dc.authorscopusid57225437022
dc.authorwosidTuna, Metin/ABA-4295-2020
dc.contributor.authorKaya, Çağlar
dc.contributor.authorTiryaki, İskender
dc.contributor.authorSarı, Ugur
dc.contributor.authorTuna, Metin
dc.date.accessioned2022-05-11T14:45:54Z
dc.date.available2022-05-11T14:45:54Z
dc.date.issued2020
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.description.abstractThis study was initiated to reveal genetic relationship of 25 tef (Eragrostis tef (Zucc.) accessions by using 10 SSR markers and to determine DNA content variation by using flow cytometer analysis. Ten markers produced a total of 18 alleles and 11 of those were polymorphic. The mean polymorphism rate was 66.6%. The highest polymorphism information content value was obtained from marker CNLTs370 with 0.69 while markers CNTLs11 and CNTLs133 produced monomorphic bands only. UPGMA analysis divided 25 tef genotypes into three main clades. The accessions PI193511 and PI195934 were distinctly separated from the others. No ploidy differences were determined among the 25 tef accessions. 2C mean nuclear DNA content ranged from 1.406 pg to 1.510 with mean of 1.460 pg. The results of this study indicated that SSR markers successfully determined genetic relationship of 25 tef accession although they had a low rate of polymorphism. This study also revealed that available tef related SSR markers should be optimized before use and their efficiency may vary based on tef genotypes or accessions used.
dc.description.sponsorshipCanakkale Onsekiz Mart University (COMU, BAP Grant)Canakkale Onsekiz Mart University [FYL-2019-2834]
dc.description.sponsorshipThe financial support of this study was provided by Canakkale Onsekiz Mart University (COMU, BAP Grant #: FYL-2019-2834).
dc.identifier.doi10.1007/s11033-020-05537-w
dc.identifier.endpage4463
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue6en_US
dc.identifier.pmid32447499
dc.identifier.scopus2-s2.0-85085372290
dc.identifier.scopusqualityQ2
dc.identifier.startpage4455
dc.identifier.urihttps://doi.org/10.1007/s11033-020-05537-w
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10177
dc.identifier.volume47
dc.identifier.wosWOS:000535099300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTuna, Metin
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectTef
dc.subjectDNA
dc.subjectSSR
dc.subjectPloidy
dc.subjectFlow cytometry
dc.subjectLinkage Map
dc.subjectGenome Size
dc.subjectDiversity
dc.subjectSequence
dc.subjectCereal
dc.subjectLines
dc.subjectPcr
dc.titleGenetic relationship and nuclear dna content variation in Tef [Eragrostis tef (Zucc.) Trotter] accessions
dc.title.alternativeEragrostis tef (Zucc.) Trotter] accessions
dc.typeArticle

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