A label-free electrochemical biosensor for highly sensitive detection of GM2A based on gold nanoparticles/conducting amino-functionalized thiophene polymer layer

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dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorAydin, Muhammet
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-10-29T17:58:29Z
dc.date.available2024-10-29T17:58:29Z
dc.date.issued2023
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractIn this study, a new immunosensor based on an indium tin oxide (ITO) substrate functionalized with gold nanoparticles (GNPs)/amino-functionalized thiophene polymer P(ThiAmn) multilayer was fabricated for impedimetric determination of GM2 activator protein (GM2A). To engineer the biosensor, a relatively simple approach composed of GNPs electrodeposition and ThiAmn electropolymerization was utilized. The use of GNPs and P(ThiAmn) increased the substrate surface area, which was beneficial to immobilize a large amount of antiGM2A biorecognition elements. The designed bifunctional layer served as a promising matrix material and provided an innovative sensor fabrication. Electrochemical techniques were employed to investigate the specific immuno-interaction process between biorecognition anti-GM2A antibodies and GM2A antigens. Apart from these techniques, spectral techniques were utilized for the characterization of different modified electrode surfaces. Under optimum conditions, GM2A was determined in a linear concentration range from 0.0185 to 111 pg/mL with a detection limit (LOD) of 5.8 fg/mL by electrochemical impedance spectroscopy (EIS) technique. This biosensor exhibited good reproducibility, long storage-stability, and excellent specificity for GM2A antigens. Additionally, this immunosensor was applied to quantify GM2A in commercial serum samples, and satisfactory results were obtained.
dc.description.sponsorshipScientific and Technological Research Council; [NKUBAP.00]
dc.description.sponsorshipThis study was funded by the Scientific and Technological Research Council (NKUBAP.00. GA.21.328) of Tekirdag ? Namik Kemal University.
dc.identifier.doi10.1016/j.snb.2023.134025
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-85161686997
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.snb.2023.134025
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14348
dc.identifier.volume392
dc.identifier.wosWOS:001058587900001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGM2 activator protein
dc.subjectLung carcinoma
dc.subjectLabel-free analysis
dc.subjectElectrochemical determination
dc.titleA label-free electrochemical biosensor for highly sensitive detection of GM2A based on gold nanoparticles/conducting amino-functionalized thiophene polymer layer
dc.typeArticle

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