A label-free electrochemical biosensor for highly sensitive detection of GM2A based on gold nanoparticles/conducting amino-functionalized thiophene polymer layer
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Sa
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, a new immunosensor based on an indium tin oxide (ITO) substrate functionalized with gold nanoparticles (GNPs)/amino-functionalized thiophene polymer P(ThiAmn) multilayer was fabricated for impedimetric determination of GM2 activator protein (GM2A). To engineer the biosensor, a relatively simple approach composed of GNPs electrodeposition and ThiAmn electropolymerization was utilized. The use of GNPs and P(ThiAmn) increased the substrate surface area, which was beneficial to immobilize a large amount of antiGM2A biorecognition elements. The designed bifunctional layer served as a promising matrix material and provided an innovative sensor fabrication. Electrochemical techniques were employed to investigate the specific immuno-interaction process between biorecognition anti-GM2A antibodies and GM2A antigens. Apart from these techniques, spectral techniques were utilized for the characterization of different modified electrode surfaces. Under optimum conditions, GM2A was determined in a linear concentration range from 0.0185 to 111 pg/mL with a detection limit (LOD) of 5.8 fg/mL by electrochemical impedance spectroscopy (EIS) technique. This biosensor exhibited good reproducibility, long storage-stability, and excellent specificity for GM2A antigens. Additionally, this immunosensor was applied to quantify GM2A in commercial serum samples, and satisfactory results were obtained.
Açıklama
Anahtar Kelimeler
GM2 activator protein, Lung carcinoma, Label-free analysis, Electrochemical determination
Kaynak
Sensors and Actuators B-Chemical
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
392
