Quantification of Trypsin Activity by a New Biosensing System Based on the Enzymatic Degradation and the Destructive Nature of Trypsin
Yükleniyor...
Dosyalar
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Trypsin is an enzyme that is included in the hydrolysis class of enzymes. Trypsin catalyzes the cleavage of proteins from a serine residue and is found in the digestive system of vertebrates. The cystic fibrosis, which is an autosomal recessive disorder, is associated with the trypsin deficiency. The meconium ileus is caused by this deficiency. Beside the clinical significance of trypsin, it is also used in many industrial applications, such as tissue culture labs, milk industry, proteomics, and different kinds of food production processes. In this study, a glucose oxidase based electrochemical biosensing system was constructed for sensitive and accurate determination of trypsin activity in different samples. A novel working principle was operated in the reported biosensor, which is based on the trypsin degradation ratio in the bioactive layer of the biosensor. Detailed optimization and characterization experiments were carried out to obtain the best electrochemical signals. The system introduced a linear determination range for trypsin activity between 6.65 and 33.25 U/ml. The biosensor was also used in the analysis of the trypsin activities of real samples.
Açıklama
Anahtar Kelimeler
Trypsin, Biosensing, Glucose oxidase, Enzyme activity, Electrochemical impedance spectroscopy, Mass-Spectrometry, Label-Free, Liquid-Chromatography, Gold Nanoparticles, Tissue Homogenate, Electron-Transfer, Diagnosis, Peptides, Proteins, Assay
Kaynak
International Journal of Peptide Research and Therapeutics
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
23
Sayı
3
