Detection of Parathyroid Hormone Using an Electrochemical Impedance Biosensor Based on PAMAM Dendrimers

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dc.authorscopusid23101201700
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorÖzcan, Hakkı Mevlüt
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:30:51Z
dc.date.available2022-05-11T14:30:51Z
dc.date.issued2015
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractThis paper presents a novel hormone-based impedimetric biosensor to determine parathyroid hormone (PTH) level in serum for diagnosis and monitoring treatment of hyperparathyroidism, hypoparathyroidism and thyroid cancer. The interaction between PTH and the biosensor was investigated by an electrochemical method. The biosensor was based on the gold electrode modified by 12-mercapto dodecanoic (12MDDA). Antiparathyroid hormone (anti-PTH) was covalently immobilized on to poly amidoamine dendrimer (PAMAM) which was bound to a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) couple, self-assembled monolayer structure from one of the other NH2 sites. The immobilization of anti-PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscope techniques. After the optimization studies of immobilization materials such as 12MDDA, EDC-NHS, PAMAM, and glutaraldehyde, the performance of the biosensor was investigated in terms of linearity, sensitivity, repeatability, and reproducibility. PTH was detected within a linear range of 10-60 fg/mL. Finally the described biosensor was used to monitor PTH levels in artificial serum samples. (C) 2015 American Institute of Chemical Engineers
dc.description.sponsorshipTrakya University Scientific Research FoundationTrakya University [TUBAP-2001/195]; TUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipSupport from Trakya University Scientific Research Foundation (Project Number: TUBAP-2001/195) and TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) are gratefully acknowledged.
dc.identifier.doi10.1002/btpr.2060
dc.identifier.endpage822
dc.identifier.issn8756-7938
dc.identifier.issn1520-6033
dc.identifier.issue3en_US
dc.identifier.pmid25683333
dc.identifier.scopus2-s2.0-84931575696
dc.identifier.scopusqualityQ2
dc.identifier.startpage815
dc.identifier.urihttps://doi.org/10.1002/btpr.2060
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7194
dc.identifier.volume31
dc.identifier.wosWOS:000356670700025
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiotechnology Progress
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectparathyroid hormone
dc.subjectbiosensor
dc.subjectPAMAM
dc.subjectelectrochemical impedance spectroscopy
dc.subject12-mercapto dodecanoic
dc.subjectPrimary Hyperparathyroidism
dc.subjectMultilayer Films
dc.subjectGlands
dc.subjectImmobilization
dc.subjectSpectroscopy
dc.subjectImmunoassay
dc.subjectCalcium
dc.subjectSensors
dc.titleDetection of Parathyroid Hormone Using an Electrochemical Impedance Biosensor Based on PAMAM Dendrimers
dc.typeArticle

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