Marker assisted selection (MAS) for downy mildew resistance in grapevines using Rpv3.1 associated markers

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dc.authorscopusid15757272300
dc.authorscopusid57641586100
dc.authorscopusid57209738441
dc.authorscopusid57210587132
dc.authorscopusid7006798815
dc.authorscopusid34573824100
dc.contributor.authorAkkurt, Murat
dc.contributor.authorŞenses, İrem
dc.contributor.authorAktürk, Burak
dc.contributor.authorTozlu, İlhami
dc.contributor.authorÖzer, Nuray
dc.contributor.authorUzun, Hail Ibrahim
dc.date.accessioned2023-04-20T08:02:24Z
dc.date.available2023-04-20T08:02:24Z
dc.date.issued2022
dc.departmentFakülteler, Ziraat Fakültesi, Bitki Koruma Bölümü
dc.description.abstractPowdery mildew and downy mildew are primary fungal diseases that cause significant damage in viticulture. Therefore, breeding powdery and/or downy mildew resistance is one of the priority subjects in grapevine breeding programs. This study aims to conduct early-selection by marker assisted selection (MAS) method among 869 genotypes obtained through crossbreeding 'Alphonse Lavallee' x 'Regent' cultivars using the markers (GF18-06 and GF18-08) associated with downy mildew resistance gene region Rpv3.1 to develop new grapevine cultivars resistant to downy mildew caused by Plasmopara viticola. A total of 869 hybrid plants which were obtained after crossing 'Alphonse Lavallee' x 'Regent' in a 3-year breeding program were used in the study. The hybrid plants were scored for the resistance level based on their sporulation intensity after artificial inoculation of P. viticola. DNA samples of the hybrid plants were amplified with GF18-06 and GF1808 markers in Polymerase Chain Reaction (PCR) for MAS. The alleles which were associated to Rpv3.1 resistance locus and the results of resistance scoring were compared, and the applicability of the markers in MAS was verified. It was determined that the GF18-08/410 bp marker can be used successfully for MAS. Gf 18-06 marker 385 bp, 390 bp and 407 bp gave false positive results in our population, respectively 8.86%, 9.02% and 37.94%. Therefore, this may limit its use for MAS.
dc.description.sponsorshipNational Scientific and Technological Research Council of Turkey [1150176]
dc.description.sponsorshipThis work was supported by the National Scientific and Technological Research Council of Turkey (grant number 1150176).
dc.identifier.doi10.15835/nbha50112708
dc.identifier.issn0255-965X
dc.identifier.issn1842-4309
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85128811098
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.15835/nbha50112708
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10900
dc.identifier.volume50
dc.identifier.wosWOS:000779788900015
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÖzer, Nuray
dc.language.isoen
dc.publisherUniv Agr Sci & Veterinary Med Cluj-Napoca
dc.relation.ispartofNotulae Botanicae Horti Agrobotanici Cluj-Napoca
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGrapevine
dc.subjectMarker Assisted Selection
dc.subjectPlasmopora Viticola
dc.subjectRpv3
dc.subjectFungal Disease Resistance
dc.subjectPlasmopara-Viticola
dc.subjectLocus
dc.subjectNecator
dc.subjectRegent
dc.subjectRpv10
dc.titleMarker assisted selection (MAS) for downy mildew resistance in grapevines using Rpv3.1 associated markers
dc.typeArticle

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