Protective effects of Acetobacter ghanensis against gliadin toxicity in intestinal epithelial cells with immunoregulatory and gluten-digestive properties

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dc.authorscopusid57190974099
dc.authorscopusid57216912962
dc.authorscopusid56685922800
dc.authorscopusid24483063300
dc.authorscopusid36608599700
dc.authorscopusid9242258800
dc.contributor.authorDoğuer, Çaglar
dc.contributor.authorAkalan, Hande
dc.contributor.authorDemirok, Nazan Tokatlı
dc.contributor.authorErdal, Berna
dc.contributor.authorMete, Rafet
dc.contributor.authorBilgen, Türker
dc.date.accessioned2023-04-20T08:01:14Z
dc.date.available2023-04-20T08:01:14Z
dc.date.issued2022
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.description.abstractPurpose The aim of this study was to establish whether Acetobacter ghanensis, the probiotic characteristics of which were evaluated previously, attenuates gliadin-induced toxicity in intestinal epithelial cells with gluten-digestive and immunoregulatory properties. Methods A co-culture model of human intestinal epithelial cell (Caco-2) monolayers on top of peripheral blood mononuclear cells (PBMCs) obtained from patients with celiac disease (CD) was established. The gluten-digestive properties of A. ghanensis were determined by checking bacterial growth in a medium containing gluten as the main nitrogen source. The mRNA levels of genes encoding TJ-associated proteins were measured by quantitative real-time PCR (qRT-PCR). The concentrations of IL-6 and TNFac were determined by enzyme-linked immunosorbent assay (ELISA). Results We found that PT-gliadin disrupted intestinal barrier integrity by modulating the expression of TJ-associated genes encoding zonulin (increased by similar to 60%), zonula occludens-1 (ZO-1) (decreased by similar to 22%), and occludin (decreased by similar to 28%) in Caco-2 cells. Furthermore, PT-gliadin treatment in Caco-2 cells was associated with increased concentrations of IL-6 (similar to 1.6-fold) and TNFac (similar to twofold) from PBMCs. These modulatory effects of PT-gliadin, however, were suppressed when Caco-2 cells were subjected to A. ghanensis in the presence of PT-gliadin. As a factor underlying these protective effects, we showed that A. ghanensis could digest gluten peptides. Conclusions To our knowledge, the current study is the first to demonstrate that A. ghanensis improves intestinal barrier functions by attenuating the modulatory effects of PT-gliadin with immunoregulatory and gluten-digestive properties.
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu [218S759]
dc.description.sponsorshipThis study is funded by Turkiye Bilimsel ve Teknolojik Arastirma Kurumu, 218S759, Caglar Doguer
dc.identifier.doi10.1007/s00394-022-03015-6
dc.identifier.issn1436-6207
dc.identifier.issn1436-6215
dc.identifier.pmid36175797
dc.identifier.scopus2-s2.0-85139099468
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s00394-022-03015-6
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10812
dc.identifier.wosWOS:000863147400002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDoğuer, Çaglar
dc.institutionauthorAkalan, Hande
dc.institutionauthorDemirok, Nazan Tokatli
dc.institutionauthorErdal, Berna
dc.institutionauthorMete, Rafet
dc.institutionauthorBilgen, Turker
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartofEuropean Journal of Nutrition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcetobacter Ghanensis
dc.subjectProbiotics
dc.subjectCeliac Disease
dc.subjectCaco-2 Cells
dc.subjectTissue Transglutaminase
dc.subjectCeliac-Disease
dc.subjectBarrier Function
dc.subjectCaco-2 Cells
dc.subjectModel
dc.subjectRelease
dc.subjectZonulin
dc.subjectInterleukin-15
dc.subjectPermeability
dc.subjectModulation
dc.titleProtective effects of Acetobacter ghanensis against gliadin toxicity in intestinal epithelial cells with immunoregulatory and gluten-digestive properties
dc.typeArticle

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