Protective effects of quercetin against apoptosis and oxidative stress in streptozotocin-induced diabetic rat testis
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Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
Pergamon-Elsevier Science Ltd
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
The aim of this study was to investigate the protective effect of quercetin (QE) on oxidative stress, apoptosis, and cell proliferation in the rat testis after streptozotocin (STZ)-induced diabetes. Diabetes was induced by a single intraperitoneal injection of STZ (50 mg/kg). The rats in the QE-treated group were given QE (15 mg/kg) once a day intraperitoneally for 8 weeks starting 3 days prior to STZ injection. At the end of the study, all animals were sacrificed. Testis tissues and blood samples were collected for histopathologic and biochemical analysis. QE treatment significantly decreased the elevated tissue malondialdehyde (MDA) levels and increased the reduced superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzyme activities in testis tissues samples. The QE-treated rats in the diabetic group showed an improved histologic appearance and serum testosterone levels. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end-labeling (TUNEL) and there was a rise in the expression of proliferating cell nuclear antigen (PCNA) in testis tissues of QE-treated rats in the diabetic group. These results suggest that administration of QE is a potentially beneficial agent to reduce testicular damage in diabetic rats by decreasing oxidative stress. Crown Copyright (c) 2011 Published by Elsevier Ltd. All rights reserved.
Description
Keywords
Experimental diabetes, Testis, Quercetin, TUNEL, Antioxidant, Testosteron, Cell Nuclear Antigen, Induced Nephrotoxicity, Malondialdehyde Levels, Seminiferous Tubules, Superoxide-Dismutase, Lipid-Peroxidation, Testicular Tissue, Free-Radicals, Antioxidant, Flavonoids
Journal or Series
Food and Chemical Toxicology
WoS Q Value
Q1
Scopus Q Value
Q1
Volume
50
Issue
45385
