A sensitive and disposable indium tin oxide based electrochemical immunosensor for label-free detection of MAGE-1

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dc.authorscopusid56106371600
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorDemirbakan, Burcak
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:30:56Z
dc.date.available2022-05-11T14:30:56Z
dc.date.issued2017
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractMAGE-1 (MAGE, for melanoma antigen), was identified by virtue of its processing and cell surface expression as a tumor-specific peptide bound to major histocompatibility complexes which was reactive with autolytic T cells. 3-Glycidoxypropyltrimethoxysilane (3-GOPS) is frequently employed for the preparation of dense heterometal hybrid polymers which are used, e.g., for hard coatings of organic polymers and contact lens materials in the optical industry. In this study, we have improved a new immunological biosensor with indium tin oxide (ITO). Then, Anti-MAGE-1 antibody was covalently immobilized with 3-GOPS which formed a self-assembled monolayers (SAMs) on modified ITO electrodes. Analytical characteristics such as square wave voltammetry, linear determination range, repeatability, reproducibility and regeneration of biosensors are determined. All characterization steps are monitored by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV). The developed biosensor has wide determination range (0.5 fg-15 fg/mL). To investigate long shelf life of the fabricated biosensor, the immunosensors were stored at 4 degrees C for periods ten weeks. Futhermore, binding kinetics of MAGE1 to antiMAGE-1 is monitored by single frequency technique in real time. Additionally, Kramer's-Kronig transform was used to understand whether the impedance spectra of biosensor system are affected from the variation that occurred because of external factor. Morphological characteristics of constructed biosensor were observed by scanning electron microscopy. Real human serum samples were also analyzed by the proposed biosensor, successfully. A commercial ELISA kit was also used as a reference method to validate the results obtained by the biosensor. Finally, this biosensor was tried in real blood sample and that showed it could be utilized in clinical applications. This biosensor can be preferred due to it has a wide linear range and it can be prepared easily.
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678]
dc.description.sponsorshipWe are grateful for the financial supports of this research from The Scientific and Technological Research Council of Turkey (Project number: 113 Z 678)
dc.identifier.doi10.1016/j.talanta.2017.03.076
dc.identifier.endpage169
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid28411807
dc.identifier.scopus2-s2.0-85016389011
dc.identifier.scopusqualityQ1
dc.identifier.startpage163
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2017.03.076
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7242
dc.identifier.volume169
dc.identifier.wosWOS:000401206700024
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDemirbakan, Burcak
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject3-Glycidoxypropyltrimethoxysilane (3-GOPS)
dc.subjectMAGE-1
dc.subjectRegeneration
dc.subjectSquare wave voltammetry (SWV)
dc.subjectElectrochemical impedance spectroscopy (EIS)
dc.subjectDirect Electrodeposition
dc.subjectGold Nanoparticles
dc.subjectUric-Acid
dc.subjectAntigen
dc.subjectPerformance
dc.subjectFabrication
dc.subjectProgression
dc.subjectFrequency
dc.subjectDopamine
dc.subjectGraphene
dc.titleA sensitive and disposable indium tin oxide based electrochemical immunosensor for label-free detection of MAGE-1
dc.typeArticle

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