Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1?, Chondroadherin, and COL2A1?

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dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorAkyuva, Yener
dc.contributor.authorDoğan, Mustafa
dc.contributor.authorErdem, İlknur
dc.date.accessioned2022-05-11T14:10:02Z
dc.date.available2022-05-11T14:10:02Z
dc.date.issued2018
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Enfeksiyon Hastalıkları Ana Bilim Dalı
dc.description.abstractAim: In this study, it was firstly aimed to investigate the effect of Daptomycin (DAP) on the proliferation in Vancomycin (VCM)-administered primary chondrocyte cultures and non-drug-administered primary chondrocyte cultures. Our second objective was to investigate the effects of DAP and VCM on the NP-specific marker protein chondroadherin (CHAD), which is associated with spinal cord and dorsal column growth, on the transcription factor-1 alpha (HIF-1?), which is induced by hypoxia, and on a type II collagen (COL2A1), which is also known to play a significant role in the development of extracellular matrix, at the pharmaco-molecular level.Material and Methods: Standard human primary chondrocyte cultures were established. DAP and VCM were added to the samples. In all groups, molecular analysis was performed at 0th, 24th and 48th hours. In addition, the surface morphology of the cells was evaluated.Results: Changes in cell morphology and cell death in cultures were observed 24 hours after administration of antibiotics to cell cultures. It was observed that drug administration was associated with the cell viability and that cell viability rate for two antibiotics was similar at the 0th and 48th hours. The expression of three genes decreased at the 24th hour in the experimental group where DAP was administered.Conclusion: Thanks to this molecular-based research, it should not be forgotten that DAP and VCM active pharmacological agents, especially used in the treatment of Methicillin-resistant Staphylococcus aureus induced surgical infections, have a negative effect on human chondrocyte and ECM components.
dc.identifier.doi10.5455/annalsmedres.2018.06.110
dc.identifier.endpage762
dc.identifier.issn2636-7688
dc.identifier.issn2636-7688
dc.identifier.issue4en_US
dc.identifier.startpage756
dc.identifier.trdizinidTWprMk56WXlNZz09
dc.identifier.urihttps://doi.org/10.5455/annalsmedres.2018.06.110
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWprMk56WXlNZz09
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5260
dc.identifier.volume25
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorKaraarslan, Numan
dc.institutionauthorŞirin, Duygu Yaşar
dc.institutionauthorDoğan, Mustafa
dc.institutionauthorErdem, İlknur
dc.language.isoen
dc.relation.ispartofAnnals of Medical Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiyoloji
dc.subjectDiş Hekimliği
dc.subjectAlerji
dc.subjectAnatomi ve Morfoloji
dc.subjectAndroloji
dc.subjectAnestezi
dc.subjectOdyoloji ve Konuşma-Dil Patolojisi
dc.subjectBiyokimya ve Moleküler Biyoloji
dc.subjectBiyofizik
dc.subjectBiyoteknoloji ve Uygulamalı Mikrobiyoloji
dc.subjectKalp ve Kalp Damar Sistemi
dc.subjectHücre Biyolojisi
dc.subjectKlinik Nöroloji
dc.subjectYoğun Bakım
dc.subjectTıp
dc.subjectDermatoloji
dc.subjectAcil Tıp
dc.subjectEndokrinoloji ve Metabolizma
dc.subjectGastroenteroloji ve Hepatoloji
dc.subjectGeriatri ve Gerontoloji
dc.subjectSağlık Bilimleri ve Hizmetleri
dc.subjectHematoloji
dc.subjectİmmünoloji
dc.subjectEnfeksiyon Hastalıkları
dc.subjectTamamlayıcı ve Entegre Tıp
dc.subjectTıbbi Etik
dc.subjectTıbbi İnformatik
dc.subjectTıbbi Laboratuar Teknolojisi
dc.subjectGenel ve Dahili Tıp
dc.subjectAdli Tıp
dc.subjectTıbbi Araştırmalar Deneysel
dc.subjectMikrobiyoloji
dc.subjectNörolojik Bilimler
dc.subjectKadın Hastalıkları ve Doğum
dc.subjectOnkoloji
dc.subjectGöz Hastalıkları
dc.subjectOrtopedi
dc.subjectKulak
dc.subjectBurun
dc.subjectBoğaz
dc.subjectPatoloji
dc.subjectPediatri
dc.subjectPeriferik Damar Hastalıkları
dc.subjectFizyoloji
dc.subjectTemel Sağlık Hizmetleri
dc.subjectPsikiyatri
dc.subjectHalk ve Çevre Sağlığı
dc.subjectRadyoloji
dc.subjectNükleer Tıp
dc.subjectTıbbi Görüntüleme
dc.subjectRehabilitasyon
dc.subjectSolunum Sistemi
dc.subjectRomatoloji
dc.subjectSpor Bilimleri
dc.subjectCerrahi
dc.subjectTransplantasyon
dc.subjectTropik Tıp
dc.subjectÜroloji ve Nefroloji
dc.subjectFarmakoloji ve Eczacılık
dc.subjectToksikoloji
dc.titleDoes transcription factor, induced by daptomycin and vancomycin, affect HIF-1?, Chondroadherin, and COL2A1?
dc.typeArticle

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