Biyopolimerler ile uygulanan lökositten fakir veya lökositten zengin trombositten zengin plazmanın kondrosit proliferasyonunda konvansiyonel trombositten zengin plazma uygulamalarına üstünlüğü var mıdır?
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Dosyalar
Tarih
2017
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Cilt Başlığı
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada trombositten zengin plazma (TZP) içeriğindeki lökosit konsantrasyonunun ve TZP'nin ilaç taşıyıcı sistem kullanılarak uygulanmasının in vitro koşullarda kondrosit proliferasyonu üzerine olası etkileri araştırıldı.Hastalar ve yöntemler: Medikal veya konservatif tedaviye yanıt vermemiş ve total diz artroplastisi geçirmiş dokuz erkek hastanın (ort. yaş 65 yıl; dağılım 49-81 yıl) kanı iki formülasyon hazırlamak üzere kullanıldı: Düşük konsantrasyon lökositli TZP (2000-4000 lökosit/µL) saf TZP (S-TZP) olarak, yüksek konsantrasyon lökositli TZP (9000-11000 lökosit/µL) ise lökositten zengin TZP (L-TZP) olarak belirlendi. Numuneler kontrol grubu (grup 1), direkt S-TZP uygulanan kondrosit kültürleri (grup 2), direkt L-TZP uygulanan kondrosit kültürleri (grup 3), S-TZP uygulanan hidrojel ile kültürlenen kondrositler (grup 4) ve L-TZP uygulanan hidrojel ile kültürlenen kondrositler (grup 5) olmak üzere beş gruba ayrıldı. Tüm gruplarda hücre morfolojisi, canlılık ve proliferasyon bir prekondrosit belirteci olan evreye özgü embriyonik antijen 1'in (SSEA-1) ifadesi ile karşılaştırıldı.Bulgular: Maksimum hücre proliferasyonu ve SSEA-1 ifadesi grup 4'te gerçekleşti; SSEA-1 ifadesi ve hücre proliferasyonu arasında istatistiksel olarak anlamlı ilişki vardı.Sonuç: Çalışmamız TZP'nin lökosit konsantrasyonunun ve hidrojel gibi taşıyıcı sistemlerin önemini ve taşıyıcı sistem ile uygulanan L-TZP'nin kıkırdak hasarının tedavisinde TZP'nin konvansiyonel uygulamalarından daha etkin olduğunu gösterdi
Objectives: This study aims to investigate the possible effects of leukocyte concentration in the content of platelet-rich plasma (PRP) and the administration of PRP using a drug delivery system on chondrocyte proliferation in vitro conditions.Patients and methods: Blood from nine male patients (mean age 65 years; range 49 to 81 years) with advanced stage osteoarthritis who had not responded to medical or conservative treatments and underwent total knee arthroplasty was used to prepare two formulations: PRP with low concentration leukocytes (2000-4000 leukocytes/µL) was designated as pure PRP (P-PRP), whereas PRP with high concentration leukocytes (9000-11000 leukocytes/µL) as leukocyte-rich PRP (L-PRP). Samples were divided into five groups as control group (group 1), chondrocyte cultures with P-PRP applied directly (group 2), chondrocyte cultures with L-PRP applied directly (group 3), chondrocytes co-cultured with P-PRP applied hydrogel (group 4), and chondrocytes co-cultured with L-PRP applied hydrogel (group 5). In all groups; cell morphology, viability and proliferation were compared with the expression of stagespecific embryonic antigen-1 (SSEA-1), a precondrocyte marker.Results: Maximum cell proliferation and SSEA-1 expression occurred in group 4, with a statistically significant correlation between SSEA-1 expression and cell proliferation.Conclusion: Our study showed the importance of leukocyte concentration of PRP and efficiency of delivery systems such as hydrogel and that L-PRP administered with a delivery system is more efficient than conventional applications of PRP in the treatment of cartilage damage
Objectives: This study aims to investigate the possible effects of leukocyte concentration in the content of platelet-rich plasma (PRP) and the administration of PRP using a drug delivery system on chondrocyte proliferation in vitro conditions.Patients and methods: Blood from nine male patients (mean age 65 years; range 49 to 81 years) with advanced stage osteoarthritis who had not responded to medical or conservative treatments and underwent total knee arthroplasty was used to prepare two formulations: PRP with low concentration leukocytes (2000-4000 leukocytes/µL) was designated as pure PRP (P-PRP), whereas PRP with high concentration leukocytes (9000-11000 leukocytes/µL) as leukocyte-rich PRP (L-PRP). Samples were divided into five groups as control group (group 1), chondrocyte cultures with P-PRP applied directly (group 2), chondrocyte cultures with L-PRP applied directly (group 3), chondrocytes co-cultured with P-PRP applied hydrogel (group 4), and chondrocytes co-cultured with L-PRP applied hydrogel (group 5). In all groups; cell morphology, viability and proliferation were compared with the expression of stagespecific embryonic antigen-1 (SSEA-1), a precondrocyte marker.Results: Maximum cell proliferation and SSEA-1 expression occurred in group 4, with a statistically significant correlation between SSEA-1 expression and cell proliferation.Conclusion: Our study showed the importance of leukocyte concentration of PRP and efficiency of delivery systems such as hydrogel and that L-PRP administered with a delivery system is more efficient than conventional applications of PRP in the treatment of cartilage damage
Açıklama
Anahtar Kelimeler
Ortopedi
Kaynak
Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.)
WoS Q Değeri
Scopus Q Değeri
Cilt
28
Sayı
3
