Functionalized magnetic nanoparticles for electrochemical magneto biosensing of PSMA cancer biomarker

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dc.authoridAYDIN, MUHAMMET/0000-0002-8716-9970
dc.contributor.authorAydin, Muhammet
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-10-29T17:58:33Z
dc.date.available2024-10-29T17:58:33Z
dc.date.issued2024
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractA novel platform on which anti-prostate specific membrane antigen (PSMA) antibodies were immobilized on the core-shell structure of iron oxide (Fe3O4)/6-phosphonohexanoic acid (6Pha) nanoparticles was developed for the specific detection of PSMA. The Fe3O4@SiO2@6Pha nanoparticles were selected as an immune sensing platform, and external magnetic force provided separation of the target PSMA antigen in biological samples. The core-shell Fe3O4@SiO2@6Pha nanoparticles conjugated with anti-PSMA antibodies were utilized for PSMA concentration measurement. The analysis strategy was based on the direct binding of anti-PSMA antibodies to the target PSMA antigens. Different electrochemical analysis approaches were utilized to characterize the biosensing system. The differences in impedimetric signals before and after interaction with PSMA antigens were associated directly with the PSMA concentration. Under optimized conditions, the linear range of 7.5-1250 pg mL-1, the detection limit of 2.21 pg mL-1, and the quantification limit of 7.36 pg mL-1 were obtained. The applicability of the immunosensor was tested on diluted human serum samples, and satisfactory results were obtained. The results illustrate that a reliable, robust, selective, and simple detection strategy was developed for further clinical diagnosis. A novel platform on which anti-prostate specific membrane antigen (PSMA) antibodies were immobilized on the core-shell structure of iron oxide (Fe3O4)/6-phosphonohexanoic acid (6Pha) nanoparticles was developed for the specific detection of PSMA.
dc.description.sponsorshipTrkiye Bilimsel ve Teknolojik Arascedil;timath;rma Kurumu [TUBITAK 222Z224]; Scientific and Technological Research Council of Turkey
dc.description.sponsorshipThe authors thank to the Scientific and Technological Research Council of Turkey (TUBITAK 222Z224).
dc.identifier.doi10.1039/d4nj00274a
dc.identifier.endpage5781
dc.identifier.issn1144-0546
dc.identifier.issn1369-9261
dc.identifier.issue13en_US
dc.identifier.scopus2-s2.0-85187707567
dc.identifier.scopusqualityQ2
dc.identifier.startpage5769
dc.identifier.urihttps://doi.org/10.1039/d4nj00274a
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14384
dc.identifier.volume48
dc.identifier.wosWOS:001181982700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofNew Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectProstate-Cancer
dc.subjectSensitive Detection
dc.subjectSurface
dc.titleFunctionalized magnetic nanoparticles for electrochemical magneto biosensing of PSMA cancer biomarker
dc.typeArticle

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