Functionalized magnetic nanoparticles for electrochemical magneto biosensing of PSMA cancer biomarker
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Royal Soc Chemistry
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel platform on which anti-prostate specific membrane antigen (PSMA) antibodies were immobilized on the core-shell structure of iron oxide (Fe3O4)/6-phosphonohexanoic acid (6Pha) nanoparticles was developed for the specific detection of PSMA. The Fe3O4@SiO2@6Pha nanoparticles were selected as an immune sensing platform, and external magnetic force provided separation of the target PSMA antigen in biological samples. The core-shell Fe3O4@SiO2@6Pha nanoparticles conjugated with anti-PSMA antibodies were utilized for PSMA concentration measurement. The analysis strategy was based on the direct binding of anti-PSMA antibodies to the target PSMA antigens. Different electrochemical analysis approaches were utilized to characterize the biosensing system. The differences in impedimetric signals before and after interaction with PSMA antigens were associated directly with the PSMA concentration. Under optimized conditions, the linear range of 7.5-1250 pg mL-1, the detection limit of 2.21 pg mL-1, and the quantification limit of 7.36 pg mL-1 were obtained. The applicability of the immunosensor was tested on diluted human serum samples, and satisfactory results were obtained. The results illustrate that a reliable, robust, selective, and simple detection strategy was developed for further clinical diagnosis. A novel platform on which anti-prostate specific membrane antigen (PSMA) antibodies were immobilized on the core-shell structure of iron oxide (Fe3O4)/6-phosphonohexanoic acid (6Pha) nanoparticles was developed for the specific detection of PSMA.
Açıklama
Anahtar Kelimeler
Prostate-Cancer, Sensitive Detection, Surface
Kaynak
New Journal of Chemistry
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
48
Sayı
13
