Determination of Developmental Stages of Cryptosporidium parvum in HCT-8 Cell Culture by Differential Interference Contrast Microscopy, Giemsa and Haematoxylin-Eosin Staining Methods

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dc.contributor.authorKar, Sırrı
dc.contributor.authorGüven, Esin
dc.contributor.authorYılmazer, Nadim
dc.contributor.authorKaraer, Zafer
dc.date.accessioned2022-05-11T14:28:26Z
dc.date.available2022-05-11T14:28:26Z
dc.date.issued2011
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractThis study aimed to compare the advantages of differential interference contrast microscopy, Giemsa and haematoxylin-eosin staining techniques for detecting, visualizing and distinguishing the developmental stages of Cryptosporidium parvum in cell culture. Data showed that interference contrast microscopy and Giemsa staining have certain advantages, whereas haematoxylin-eosin staining followed by formalin fixation clearly reveals certain changes occurred in the parasite. In the investigations, it was revealed that direct microscopy is effective in accurate definition of meronts and microgamets, but staining methods can differentiate meronts only.
dc.identifier.endpage175
dc.identifier.issn1300-6045
dc.identifier.issue2en_US
dc.identifier.startpage171
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6826
dc.identifier.volume17
dc.identifier.wosWOS:000288117600002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.institutionauthorYılmazer, Nadim
dc.institutionauthorKar, Sırrı
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryptosporidium
dc.subjectCell culture
dc.subjectGiemsa
dc.subjectHaematoxylin-Eosin
dc.subjectIn-Vitro
dc.subjectOocysts
dc.subjectApoptosis
dc.subjectCultivation
dc.subjectApicomplexa
dc.subjectInfection
dc.subjectLine
dc.titleDetermination of Developmental Stages of Cryptosporidium parvum in HCT-8 Cell Culture by Differential Interference Contrast Microscopy, Giemsa and Haematoxylin-Eosin Staining Methods
dc.typeArticle

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