Determination of Developmental Stages of Cryptosporidium parvum in HCT-8 Cell Culture by Differential Interference Contrast Microscopy, Giemsa and Haematoxylin-Eosin Staining Methods
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Dosyalar
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kafkas Univ, Veteriner Fakultesi Dergisi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study aimed to compare the advantages of differential interference contrast microscopy, Giemsa and haematoxylin-eosin staining techniques for detecting, visualizing and distinguishing the developmental stages of Cryptosporidium parvum in cell culture. Data showed that interference contrast microscopy and Giemsa staining have certain advantages, whereas haematoxylin-eosin staining followed by formalin fixation clearly reveals certain changes occurred in the parasite. In the investigations, it was revealed that direct microscopy is effective in accurate definition of meronts and microgamets, but staining methods can differentiate meronts only.
Açıklama
Anahtar Kelimeler
Cryptosporidium, Cell culture, Giemsa, Haematoxylin-Eosin, In-Vitro, Oocysts, Apoptosis, Cultivation, Apicomplexa, Infection, Line
Kaynak
Kafkas Universitesi Veteriner Fakultesi Dergisi
WoS Q Değeri
Q3
Scopus Q Değeri
Cilt
17
Sayı
2
