Amperometric uric acid biosensor based on poly(vinylferrocene)-gelatin-carboxylated multiwalled carbon nanotube modified glassy carbon electrode

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dc.authorid0000-0001-5153-8319
dc.authorscopusid12784153100
dc.authorscopusid55798756500
dc.authorscopusid25422504200
dc.authorscopusid7006065730
dc.authorwosidOzturk, funda/G-9435-2014
dc.authorwosidKacar, Ceren/ABB-9363-2020
dc.contributor.authorErden, Pınar Esra
dc.contributor.authorKaçar, Ceren
dc.contributor.authorÖztürk, Funda
dc.contributor.authorKılıç, Esma
dc.date.accessioned2022-05-11T14:29:58Z
dc.date.available2022-05-11T14:29:58Z
dc.date.issued2015
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractIn this study, a new uric acid biosensor was constructed based on ferrocene containing polymer poly (vinylferrocene) (PVF), carboxylated multiwalled carbon nanotubes (c-MWCNT) and gelatin (GEL) modified glassy carbon electrode (GCE). Uricase enzyme (UOx) was immobilized covalently through N-ethyl-N'-(3-dimethyaminopropyl) carbodiimide (EDC) and N-hydroxyl succinimide (NHS) chemistry onto c-MWCNT/GEL/PVF/GCE. The c-MWCNT/GEL/PVF composite was characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. Various experimental parameters such as pH, applied potential, enzyme loading, PVF and c-MWCNT concentration were investigated in detail. Under the optimal conditions the dynamic linear range of uric acid was 2.0 x 10(-7) M - 7.1 x 10(-4) M (R=0.9993) with the detection limit low to 2.3 x 10(-8) M. With good selectivity and sensitivity, the biosensor was successfully applied to determine the uric acid in human serum. The results of the biosensor were in good agreement with those obtained from standard method. Therefore, the presented biosensor could be a good promise for practical applications in real samples. (C) 2014 Elsevier B.V. All rights reserved.
dc.description.sponsorshipAnkara University Research FundAnkara University [14L0430005]
dc.description.sponsorshipWe gratefully acknowledge the financial support of Ankara University Research Fund (Project No: 14L0430005).
dc.identifier.doi10.1016/j.talanta.2014.11.058
dc.identifier.endpage495
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid25618698
dc.identifier.scopus2-s2.0-84918519426
dc.identifier.scopusqualityQ1
dc.identifier.startpage488
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2014.11.058
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7188
dc.identifier.volume134
dc.identifier.wosWOS:000349723600066
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖztürk, Funda
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAmperometry
dc.subjectUric acid
dc.subjectBiosensor
dc.subjectPoly(vinylferrocene)
dc.subjectMediator
dc.subjectCarboxylated multiwalled carbon nanotube
dc.subjectEnzyme Electrode
dc.subjectPoly(Vinylferrocenium) Film
dc.subjectPolypyrrole Film
dc.subjectComposite Film
dc.subjectImmobilization
dc.subjectSerum
dc.subjectOxidase
dc.subjectSensor
dc.subjectGlucose
dc.subjectConstruction
dc.titleAmperometric uric acid biosensor based on poly(vinylferrocene)-gelatin-carboxylated multiwalled carbon nanotube modified glassy carbon electrode
dc.typeArticle

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