Electrochemical immunosensor for CDH22 biomarker based on benzaldehyde substituted poly(phosphazene) modified disposable ITO electrode: A new fabrication strategy for biosensors

Basit Öğe Kaydı
dc.authorscopusid55571487900
dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.contributor.authorAydın, Muhammet
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:07:23Z
dc.date.available2022-05-11T14:07:23Z
dc.date.issued2019
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA novel label-free impedimetric immunosensor was fabricated for rapid, selective and sensitive detection and quantification of Cadherin-like protein 22 (CDH22), a cancer marker, in human serum by using easy and quickly prepared disposable ITO immunoelectrode. cancer marker, in human serum by using easy and quickly prepared disposable ITO immunoelectrode. The biosensing approach implied the use of ITO electrode coated with poly(phosphazene) polymer including benzaldehyde groups attached with CDH22 antibody and CDH22 antigens. Benzaldehyde side groups containing poly(phosphazene) film coated disposable ITO electrode were utilized as an immunosensing platform and anti-CDH22 antibodies bound to aldehyde groups of benzaldehyde substituted poly(phosphazene) (P-PHP) covalently. The immunosensor modification steps and affinity interaction between anti-CDH22 antibodies and CDH22 antigens were observed by EIS and CV in the presence of the redox couple. Furthermore, antibody immobilization was followed via FTIR and Raman spectroscopy. The morphological analyses of the suggested immunosensor during the fabrication steps were carried out with SEM and AFM monitoring. All the experimental parameters affecting the construction of the immunoelectrodes were optimized. The fabricated immunosensor exhibited an excellent working performance with a wide detection linear range (0.015–2.9 pg/mL) and low limit of detection (4.4 fg/mL). Moreover, the proposed immunosensor had great reproducibility, repeatability and long-term stability. Additionally, the fabricated immunosensor was successfully used in the quantification of CDH22 in human serum without any pretreatment. © 2018 Elsevier B.V.
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK: 113 Z 678
dc.description.sponsorshipThe financial support from TÜBITAK (The Scientific and Technological Research Council of Turkey) (Grant no. 113 Z 678 ) is gratefully acknowledged.
dc.identifier.doi10.1016/j.bios.2018.10.051
dc.identifier.endpage239
dc.identifier.issn0956-5663
dc.identifier.pmid30439624
dc.identifier.scopus2-s2.0-85056230078
dc.identifier.scopusqualityQ1
dc.identifier.startpage230
dc.identifier.urihttps://doi.org/10.1016/j.bios.2018.10.051
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5089
dc.identifier.volume126
dc.identifier.wosWOS:000457659500031
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofBiosensors and Bioelectronics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBenzaldehyde substituted poly(phosphazene)
dc.subjectCadherin-like protein 22
dc.subjectCancer biomarker
dc.subjectElectrochemical impedance spectroscopy
dc.subjectAntibodies
dc.subjectAntigens
dc.subjectBiomarkers
dc.subjectBody fluids
dc.subjectDiseases
dc.subjectElectrochemical electrodes
dc.subjectElectrochemical impedance spectroscopy
dc.subjectFourier transform infrared spectroscopy
dc.subjectGlycoproteins
dc.subjectImmunosensors
dc.subjectAntibody immobilization
dc.subjectCadherin
dc.subjectCancer biomarkers
dc.subjectElectrochemical immunosensors
dc.subjectExperimental parameters
dc.subjectFabrication strategies
dc.subjectImpedimetric immunosensors
dc.subjectPhosphazenes
dc.subjectFabrication
dc.subjectaldehyde
dc.subjectbenzaldehyde
dc.subjectcadherin like protein 22
dc.subjectcadherin like protein 22 antibody
dc.subjectindium tin oxide
dc.subjectoncoprotein
dc.subjectpoly(phosphazene)
dc.subjectpolymer
dc.subjectprotein antibody
dc.subjecttumor marker
dc.subjectunclassified drug
dc.subjectcadherin
dc.subjectCDH22 protein, human
dc.subjectimmobilized antibody
dc.subjectorganophosphorus compound
dc.subjectpoly(phosphazene)
dc.subjectpolymer
dc.subjecttumor marker
dc.subjectantigen antibody reaction
dc.subjectArticle
dc.subjectatomic force microscopy
dc.subjectbinding affinity
dc.subjectchemical modification
dc.subjectcontrolled study
dc.subjectcovalent bond
dc.subjectFourier transform spectroscopy
dc.subjectimmobilization
dc.subjectimpedance spectroscopy
dc.subjectlimit of detection
dc.subjectoxidation reduction reaction
dc.subjectRaman spectrometry
dc.subjectreproducibility
dc.subjectscanning electron microscopy
dc.subjectstructure analysis
dc.subjectblood
dc.subjectchemistry
dc.subjectelectrochemical analysis
dc.subjectgenetic procedures
dc.subjecthuman
dc.subjectimmunoassay
dc.subjectimmunology
dc.subjectisolation and purification
dc.subjectneoplasm
dc.subjectprocedures
dc.subjectstandards
dc.subjectAntibodies, Immobilized
dc.subjectBiomarkers, Tumor
dc.subjectBiosensing Techniques
dc.subjectCadherins
dc.subjectElectrochemical Techniques
dc.subjectHumans
dc.subjectImmunoassay
dc.subjectLimit of Detection
dc.subjectNeoplasms
dc.subjectOrganophosphorus Compounds
dc.subjectPolymers
dc.titleElectrochemical immunosensor for CDH22 biomarker based on benzaldehyde substituted poly(phosphazene) modified disposable ITO electrode: A new fabrication strategy for biosensors
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
5089.pdf
Boyut:
4.9 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu