Electrochemical immunosensor for CDH22 biomarker based on benzaldehyde substituted poly(phosphazene) modified disposable ITO electrode: A new fabrication strategy for biosensors
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Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel label-free impedimetric immunosensor was fabricated for rapid, selective and sensitive detection and quantification of Cadherin-like protein 22 (CDH22), a cancer marker, in human serum by using easy and quickly prepared disposable ITO immunoelectrode. cancer marker, in human serum by using easy and quickly prepared disposable ITO immunoelectrode. The biosensing approach implied the use of ITO electrode coated with poly(phosphazene) polymer including benzaldehyde groups attached with CDH22 antibody and CDH22 antigens. Benzaldehyde side groups containing poly(phosphazene) film coated disposable ITO electrode were utilized as an immunosensing platform and anti-CDH22 antibodies bound to aldehyde groups of benzaldehyde substituted poly(phosphazene) (P-PHP) covalently. The immunosensor modification steps and affinity interaction between anti-CDH22 antibodies and CDH22 antigens were observed by EIS and CV in the presence of the redox couple. Furthermore, antibody immobilization was followed via FTIR and Raman spectroscopy. The morphological analyses of the suggested immunosensor during the fabrication steps were carried out with SEM and AFM monitoring. All the experimental parameters affecting the construction of the immunoelectrodes were optimized. The fabricated immunosensor exhibited an excellent working performance with a wide detection linear range (0.015–2.9 pg/mL) and low limit of detection (4.4 fg/mL). Moreover, the proposed immunosensor had great reproducibility, repeatability and long-term stability. Additionally, the fabricated immunosensor was successfully used in the quantification of CDH22 in human serum without any pretreatment. © 2018 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Benzaldehyde substituted poly(phosphazene), Cadherin-like protein 22, Cancer biomarker, Electrochemical impedance spectroscopy, Antibodies, Antigens, Biomarkers, Body fluids, Diseases, Electrochemical electrodes, Electrochemical impedance spectroscopy, Fourier transform infrared spectroscopy, Glycoproteins, Immunosensors, Antibody immobilization, Cadherin, Cancer biomarkers, Electrochemical immunosensors, Experimental parameters, Fabrication strategies, Impedimetric immunosensors, Phosphazenes, Fabrication, aldehyde, benzaldehyde, cadherin like protein 22, cadherin like protein 22 antibody, indium tin oxide, oncoprotein, poly(phosphazene), polymer, protein antibody, tumor marker, unclassified drug, cadherin, CDH22 protein, human, immobilized antibody, organophosphorus compound, poly(phosphazene), polymer, tumor marker, antigen antibody reaction, Article, atomic force microscopy, binding affinity, chemical modification, controlled study, covalent bond, Fourier transform spectroscopy, immobilization, impedance spectroscopy, limit of detection, oxidation reduction reaction, Raman spectrometry, reproducibility, scanning electron microscopy, structure analysis, blood, chemistry, electrochemical analysis, genetic procedures, human, immunoassay, immunology, isolation and purification, neoplasm, procedures, standards, Antibodies, Immobilized, Biomarkers, Tumor, Biosensing Techniques, Cadherins, Electrochemical Techniques, Humans, Immunoassay, Limit of Detection, Neoplasms, Organophosphorus Compounds, Polymers
Kaynak
Biosensors and Bioelectronics
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
126
